在流感病毒迷你核糖核蛋白复合体中,聚合酶-核蛋白-RNA的合
Huiling Kang1,2,3,4, Yunxiang Yang2, Yixiao Liu2
1Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
Nature communications
|November 4, 2025
概括
流感病毒的核糖蛋白复合体 (RNP) 经历了形状变化. 原子分辨率结构揭示了聚合酶复合体 (FluPol) 如何与不同状态的核蛋白 (NP) 和RNA相互作用,影响病毒复制.
科学领域:
- 病毒学 病毒学
- 结构生物学 结构生物学
- 分子生物学分子生物学
背景情况:
- 流感病毒核糖蛋白复合体 (RNP) 对病毒复制和转录至关重要.
- RNAP由病毒RNA (vRNA),核蛋白 (NP) 和RNA聚合酶复合体 (FluPol) 组成.
研究的目的:
- 确定不同功能状态下的流感病毒迷你RNP的原子分辨率结构.
- 阐明RNP复合体内的FluPol,NP和vRNA之间的详细相互作用.
主要方法:
- 在原子分辨率下使用冷电子显微镜 (cryo-EM).
- 在两个不同的构造状态 (进入状态和退出状态) 中分析小型RNP.
主要成果:
- 结构显示FluPol位于NP-RNA环的内部 (进入状态) 或边缘 (退出状态).
- 确定了与FluPol和vRNA促进体的特定NP (NP-0) 相互作用,其D72-K90循环插入RNA叉.
- 由NP槽形成的连续RNA结合路径,与负链RNA病毒机制一致.
- 进入状态阻止了FluPol二元化和Pol II相互作用的接口,而退出状态则暴露了它们.
结论:
- 这项研究提供了关于流感RNP中FluPol,NP和vRNA的合的详细见解.
- 观察到的构造变化表明病毒生命周期期间RNP的动态变化.
- 这些发现有助于理解流感病毒复制机制.
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