基因交换网络在细菌病原体Listeria monocytogenes中的移动DNA载体中形成桥梁
Héloïse Muller1, Odion O Ikhimiukor1, Manuela Montoya-Giraldo1
1Department of Biological Sciences, State University of New York at Albany, Albany, New York, USA.
Nature communications
|November 4, 2025
概括
李斯特菌单细胞原体中的移动遗传元素 (MGE) 形成了一个结构化的网络,促进了对抗压力耐受性和毒性基因的交换. 这种遗传交换推动了细菌的适应和病原体群体内的特征的传播.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 进化生物学 进化生物学
背景情况:
- 李斯特菌 (Listeria monocytogenes,Lm) 是一种主要的食源性病原体,可引起胃肠道和侵入性感染.
- 移动遗传元素 (MGE) 对于LM的抗微生物耐药性,毒性和适应性至关重要.
- 了解MGEs是控制LM感染的关键.
研究的目的:
- 在临床Listeria monocytogenes种群中对移动遗传元素 (MGE) 池进行表征.
- 研究不同MGE类型之间的遗传关系和交换模式.
- 识别与应激耐受性相关的基因及其在MGEs中的移动性.
主要方法:
- 从纽约州 (2000-2021) 分析了936个临床LM基因组.
- 为假定的MGEs建立一个序列同样性网络.
- 使用长读序列和全球基因组数据集进行比较分析.
主要成果:
- 确定了一个结构化的MGE网络,与类似DNA区域的社区.
- 观察到不同MGE类型 (例如,等离子体-转子体,菌体-等离子体) 之间的相互联系.
- 压力耐受性基因在等离子体和转子体中过度表现,并显示出移动性.
结论:
- MGEs之间的遗传交换显著影响了Listeria monocytogenes的物种内部变异.
- 这种相互连接的MGE网络促进了临床相关特征的快速传播.
- 了解这些交流对于预测和管理LM进化和病原性至关重要.
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