佐古伊万在精的治疗潜力:来自网络药理学,分子对接,分子动力学模拟和实验验证的见解
Mingzhao Zhang1,2, Jisheng Wang3, Junlong Feng4
1Urology, Henan Second Provincial People's Hospital, Zhengzhou, Henan, China.
Scientific reports
|November 4, 2025
概括
中国专利医药Zuogui Wan (ZGW) 显示出治疗男性不孕症的前景,而这种不孕症是由Oligoasthenozoospermia (OAS) 引起的. 它通过调节关键分子通路和减少亡来改善精子质量,Cyasterone被确定为关键活性化合物.
科学领域:
- 生殖生物学 生殖生物学
- 药理学 药理学是指药理学的学科.
- 生物信息学是一种生物信息学.
背景情况:
- 精子和精子 (OAS) 是男性不孕症的重要原因,治疗选择有限.
- 传统中医药Zuogui Wan (ZGW) 用于改善精子质量,但其潜在的分子机制尚不清楚.
研究的目的:
- 阐明Zuogui Wan (ZGW) 的分子机制,用于治疗小精 (OAS).
- 确定关键活性化合物和ZGW分子标,与OAS处理相关.
- 为了验证ZGW的治疗效果,OAS的体外和体外模型.
主要方法:
- 综合网络药理学,分子对接,分子动力学 (MD) 模拟和体外/体内实验.
- 确定了ZGW活性化合物和标,分析了缩和蛋白质-蛋白质相互作用 (PPI) 网络.
- 在大鼠OAS模型中评估了化合物-标结合,细胞增殖,基因/蛋白质表达和丸功能.
主要成果:
- 确定了182个潜在的目标,其中TP53,NF-κB1和PKC是关键的枢纽基因;突出了PI3K-AKT和MAPK信号通路.
- 发现了四种核心化合物 (Cyasterone,Betavulgarin,Kaempferol,Quercetin),其中Cyasterone表现出最强的结合和稳定性.
- ZGW显著增强了细胞增殖,改善了丸结构,精子质量,并在体外和体内减少了细胞亡.
结论:
- 通过调节亡,氧化应激和关键信号通路 (PI3K-AKT,MAPK),ZGW有效地治疗化 (OAS).
- 化合物Cyasterone显示出与核心标具有显著的结合亲和力和稳定性.
- ZGW代表了与OAS相关的男性不孕症的潜在治疗策略.
关键词:
细胞亡是细胞的亡.通过MAPK路径.分子对接是分子对接.模拟分子动力学模拟网络药理学 网络药理学在Oligoasthenozoospermia中使用.通过PI3K-AKT途径.Zuogui wan wan 的意思是什么意思更多相关视频
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