深静脉血栓症的分子复杂性初步基于ceRNA网络,scRNA-seq和AlphaFold 2的基础上进行了探索
Bao-Ze Pan1, Qing-Yu Luo1, Ming-Jun Jiang1
1Department of Vascular Surgery, The Second Affiliated Hospital, Hengyang Medical School, University of South China, No.35, Jiefang Road, Hengyang, Hunan Province, 421001, People's Republic of China.
BMC medical genomics
|November 4, 2025
概括
这项研究确定了五个关键基因 (JAK2,CD36,TNFSF13B,TLR7,PARP9) 作为深静脉血栓症 (DVT) 的潜在目标. 一个涉及circRNA,miRNA和TNFSF13B的特定调节途径也被突出显示为DVT.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 深静脉血栓症 (DVT) 是一种常见的血管疾病,具有复杂的发病因子和对调节目标的有限理解.
- 识别新的分子点对于理解DVT并发症至关重要.
研究的目的:
- 为了确定与DVT相关的独特基因.
- 调查DVT背后的复杂监管机制.
主要方法:
- 在DVT患者和健康对照中对circRNAs,miRNAs和mRNAs进行全面的转录组分析.
- 竞争性内源RNA (ceRNA) 网络模型的构建.
- 蛋白与蛋白相互作用 (PPI) 网络分析,AlphaFold 2结构预测和比较毒基因组学数据库 (CTD) 评估.
- 单细胞RNA测序 (scRNA-seq) 用于确定细胞基因表达局部化和通路作用.
主要成果:
- 鉴定了406种差异表达的circRNA,29种miRNA和154种mRNA.
- 构建ceRNA网络,揭示关键相互作用.
- 通过PPI网络分析确定了五个枢纽基因 (JAK2,CD36,TNFSF13B,TLR7,PARP9).
- scRNA-seq揭示了不同细胞类型中不同的基因表达模式和通路作用.
结论:
- 建议将JAK2,CD36,TNFSF13B,TLR7和PARP9作为DVT的潜在监管目标.
- 该途径hsa_circ_0095124/hsa-miR-3074-5p/TNFSF13B被确定为潜在的调节器在DVT的发病.
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