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CIAPIN1作为促进口腔状细胞癌进展的促进剂

Shan Yang¹, Changsheng Sun¹, Zhikai Fang¹

¹The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, People's Republic of China.

Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology

|November 5, 2025

概括

细胞因子诱导的亡抑制剂1 (CIAPIN1) 在口腔状细胞癌 (OSCC) 中被上调,并促进其恶性进展. 击败CIAPIN1会降低OSCC细胞的活力,迁移和入侵,同时增加细胞亡.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
癌症研究癌症研究

背景情况:

口腔状细胞癌 (OSCC) 是一种常见的恶性瘤,具有复杂的分子基础.
了解特定基因在OSCC发育中的作用对于确定治疗点至关重要.

研究的目的:

在OSCC中研究细胞因子诱导的亡抑制剂1 (CIAPIN1) 的表达.
阐明CIAPIN1在OSCC进展中的功能性作用,包括其对细胞活力,迁移,入侵,上皮-介质细胞过渡 (EMT) 和亡的影响.

主要方法:

在各种癌症中对CIAPIN1进行生物信息分析.
免疫组织化学 (IHC) 用于评估OSCC组织和健康对照中的CIAPIN1蛋白水平.
定量实时PCR (qRT-PCR) 和Western Blot (WB) 用于确定OSCC细胞系中的CIAPIN1mRNA和蛋白质表达.
在体外功能测试 (MTT, scratch, transwell,流动细胞计, WB) 评估CIAPIN1对OSCC细胞行为和EMT标记物的作用.

主要成果:

CIAPIN1在多种瘤中表现异常,与健康样本相比,OSCC组织中观察到显著的上调 (p < 0.001).
与正常HOK细胞相比,OSCC细胞系 (CAL-27,SAS,SCC9) 的CIAPIN1mRNA和蛋白质水平显著升高.
在SAS和SCC9细胞中,CIAPIN1的淘汰导致细胞活力,迁移和入侵的降低,以及细胞亡的增加.
抑制CIAPIN1导致N-cadherin减少和E-cadherin表达增加,表明EMT的抑制.

结论:

CIAPIN1在促进口腔状细胞癌的恶性进展方面发挥着重要作用.
在OSCC中,CIAPIN1有助于增强细胞活力,迁移,入侵和EMT.
CIAPIN1代表了口腔状细胞癌的潜在治疗标.

关键词:

美国中央情报局PIN1 在EMT中,EMT是EMT.这是OSCCCC的OSCC.灭症 (apoptosis) 是一种死亡的过程.

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