中性粒细胞蛋白作为皮结核查测试的潜在生物标志物
Mark Chambers1, Farina Karim1, Matilda Mazibuko1
1Africa Health Research Institute, Department of Basic and Translational Science, Durban, South Africa.
Frontiers in immunology
|November 5, 2025
概括
这项研究表明,测量唾液中的中性粒细胞蛋白质可以帮助查结核病 (TB). 这些生物标志物,包括S100A8/A9,在资源有限的环境中显示出对结核病快速测试的希望.
科学领域:
- 发现生物标志物的发现.
- 传染病诊断 传染病诊断 传染病诊断
- 肺部医学 肺部医学
背景情况:
- 结核病 (TB) 查需要快速和负担得起的方法,特别是在资源有限的环境中.
- 唾液是结核病检测的主要样本,含有中性粒细胞,在结核病患者中升高.
- 与中性粒细胞相关的蛋白质显示出作为结核病生物标志物的潜力.
研究的目的:
- 评估唾液中与中性粒细胞相关的蛋白质 (NGAL,S100A8/A9,PDL-1) 作为潜在的结核病查生物标志物.
- 评估这些蛋白质的性能与世界卫生组织针对结核病选试验的目标产品概况相比.
- 为了调查这些蛋白质存在于替代样本,如舌头拭子和无症状结核病病例中的存在.
主要方法:
- 使用ELISA测量了使用假定结核病例的唾液样本中的NGAL,S100A8/A9和PDL-1.
- 分析了来自南非两个不同的结核病队伍的样本.
- 在舌头拭子和无症状结核病患者中研究了蛋白质水平.
主要成果:
- 与对照组相比,在诊所诊断的结核病患者的唾液中检测到所有三种蛋白质的显著增加.
- S100A8/A9在结核病分离方面表现出高性能,其ROC AUC为0.866.
- 蛋白质水平与治疗后的细菌负载或减少没有一致的相关性;只有PDL-1在舌头拭片中被发现.
结论:
- 唾液中的中性粒细胞蛋白很容易测量,并显示出作为结核病查测试的潜力.
- 测试的蛋白质显示出结核病位测试的前景,接近世卫组织的目标.
- 需要进一步的研究来验证这些中性粒细胞蛋白为可部署的结核病查策略.
更多相关视频
09:54Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection
Published on: February 16, 2020
6.1K
08:48Separation and Fractionation of Culture Filtrate Proteins (CFPs) from Mycobacterium tuberculosis
Published on: July 11, 2025
541
相关概念视频
01:26Pulmonary Tuberculosis IV
469
Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
469
01:26Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture
557
Sputum studies are a critical part of diagnosing and treating numerous respiratory conditions. These studies involve obtaining sputum samples for analysis to identify pathogenic organisms and assess the presence of abnormal cells indicative of malignant conditions. This lesson will delve into three fundamental sputum studies: Gram Stain, Cytology, and Acid-fast Smear and Culture.
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
557