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在α-Humulene中嗅觉受体的功能冗余性调解了一个世界性的昆虫害虫的 Ovipositional 行为

Shuyuan Yao^1,2,3,4, Xuanhao Chen^1,2,3, Yi Chen^1,2,3

¹College of juncao Science and Ecology, College of Life Science, and State Key Laboratory of Agricultural and Forestry Biosecurity, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Journal of agricultural and food chemistry

|November 5, 2025

概括

钻石背使用特定的气味受体来检测宿主植物的气味,如用于产卵的α-Humulene. 阻止这些受体可以破坏的吸引力,提供新的害虫控制策略.

科学领域:

昆虫学昆虫学是一门学科.
神经科学是一个神经科学.
化学生态化学生态学

背景情况:

昆虫依靠嗅觉生存,像金刚石背 (Plutella xylostella) 这样的害虫,由于耐杀虫剂的存在,给农业带来了重大挑战.
了解P. xylostella的嗅觉机制,特别是它对宿主植物挥发性物质 (如α-Humulene) 的反应,对于开发新的控制策略至关重要.

研究的目的:

调查两个特定的嗅觉受体基因,PxOR28和PxOR31在钻石背对α-Humulene的嗅觉感知中的作用.
确定这些受体是否对于调解对α-Humulene的行为反应至关重要,包括宿主植物吸引力和卵位.

主要方法:

基因克隆和定量PCR (qPCR) 用于评估雌性天线中的*PxOR28*和*PxOR31*的表达.
通过CRISPR/Cas9基因编辑,创造出缺乏功能性*PxOR28*和*PxOR31*基因的淘汰突变者.
行为测试以评估蝶对α-Humulene的吸引力和野生类型和突变个体的卵位.

主要成果:

发现*PxOR28*和*PxOR31*在雌性天线中表达很高,对于介导对α-Humulene的吸引力至关重要.
野生类型的蝶在对α-Humulene的反应中表现出吸引力和卵置行为.
缺乏*PxOR28*和*PxOR31*的突变蝶对α-Humulene没有表现出任何行为反应,这表明这些受体之间存在功能冗余.

结论:

这项研究阐明了P. xylostella*感知宿主挥发性α-Humulene的特定嗅觉机制.
已确定的化学感知受体 (*PxOR28*和 *PxOR31*) 和气味剂α-Humulene代表了开发针对钻石背的创新和可持续害虫控制策略的潜在目标.

关键词:

这就是CRISPR/Cas9的作用.分子对接的分子对接.嗅觉受体是一种嗅觉受体.普鲁特拉克西洛斯特拉产卵偏好产卵偏好

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