在可选的多细胞真核生物中,聚基酸合成酶的全球概况
Markus Günther1, Rosa Herbst1, Nico Ueberschaar2
1Department of Paleobiotechnology, Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute, Jena D-07745, Germany.
概括
破坏Dictyostelium discoideum中的DiSfp基因停止了多基胺的产生,影响了生长,细胞运动和子的形成. 这揭示了多基基菌.
科学领域:
- 分子生物学分子生物学
- 生物化学 生化学
- 发现自然产品的发现
背景情况:
- 聚基类是各种各样的自然产品,具有重要的生物作用.
- 迪西奥斯蒂迪斯科伊德 (Dictyostelium discoideum) 拥有40个聚基因合成酶 (PKS) 基因,其功能在很大程度上是未知的.
- PKS 激活需要酸乙烯转移酶 DiSfp.
研究的目的:
- 研究Dictyostelium discoideum中DiSfp和PKS衍生代谢物的功能作用.
- 了解多基基对细胞生理和发育的贡献.
主要方法:
- 在Dictyostelium discoideum中DiSfp的基因破坏.
- 综合的表型,转录和代谢分析.
- 野生类型和突变菌株的比较代谢概况.
主要成果:
- DiSfp 干扰取消了聚基化物代谢产物的产生.
- 突变者表现出生长受损,有缺陷的巨细胞生长,异常的化学反应和减少的子形成.
- 在不同发育阶段确定了候选的多基基化物代谢物.
结论:
- 聚基基对于Dictyostelium discoideum的生长,细胞过程和发育至关重要.
- 这项研究确定了PKS机械和代谢/发育网络之间的功能联系.
- 为未来在Dictyostelium中发现多基化物提供了一个资源.
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