评估持续纳秒脉冲电场 (nsPEF) 处理作为人类牛奶消毒的非热替代方案
Yiting Wang1, Farzan Zare1,2, Elisabeth K Prabawati1
1School of Agriculture and Food Sustainability, The University of Queensland, St Lucia, Queensland, Australia.
PloS one
|November 5, 2025
概括
纳米秒脉冲电场 (nsPEF) 提供了一个低温的替代方案,可以将捐赠人乳 (DHM) 化. 虽然对大肠杆菌有效,但牛奶成分可能会提供保护,需要进一步优化以实现完全的细菌失活.
科学领域:
- 食品科学与技术 食品科学与技术
- 微生物学 微生物学
- 生物医学工程 生物医学工程
背景情况:
- 捐赠人乳 (DHM) 需要消毒以确保安全.
- 热巴氏杀菌可以降解DHM中的营养和生物活性成分.
- 对于DHM,需要使用替代的非热巴氏杀菌方法.
研究的目的:
- 调查纳秒脉冲电场 (nsPEF) 作为DHM的新消毒方法.
- 为了优化nsPEF参数,使用模仿DHM的盐溶液.
- 评估nsPEF在减少大肠杆菌在DHM中的有效性.
主要方法:
- 使用0.1%盐溶液优化nsPEF参数.
- 用大肠杆菌在各种初始度中接种的DHM的治疗.
- 显微镜分析以确定细菌的潜在保护因素.
- 在nsPEF处理过程中监测温度.
主要成果:
- 在盐溶液中以5 log CFU/mL完成大肠杆菌失活,并在8 log CFU/mL显著减少.
- nsPEF治疗实现了3-5日志CFU/mL减少大肠杆菌在DHM.
- 显微镜分析表明,牛奶脂肪球体和上皮细胞具有保护作用.
- 在nsPEF处理期间的最大温度保持在36°C以下.
结论:
- nsPEF处理是DHM消毒的有希望的低温替代方案.
- 母乳成分可能会对细菌产生对nsPEF的保护作用.
- 需要进一步的研究来优化nsPEF以提高DHM中的细菌失活.
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