Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Glycolysis: Preparatory Phase01:21

Glycolysis: Preparatory Phase

16.4K
In cellular metabolism (the complete breakdown of glucose to extract energy),  glycolysis is the first step. Glycolysis takes place in the cytoplasm of both prokaryotic and eukaryotic cells. Glucose enters heterotrophic cells in two ways. One method is through secondary active transport, where the transport takes place against the glucose concentration gradient. The other mechanism uses a group of integral proteins called GLUT proteins, also known as glucose transporter proteins. These...
16.4K
Other Glycolytic Pathways01:24

Other Glycolytic Pathways

800
The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
800
Biosynthesis of Polysaccharides01:26

Biosynthesis of Polysaccharides

539
Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
539
Energy-requiring Steps of Glycolysis01:20

Energy-requiring Steps of Glycolysis

171.1K
Glucose is the source of nearly all energy used by organisms. The first step of converting glucose into usable energy is called glycolysis. Glycolysis occurs in the cytosol of the cell over two phases: an energy-requiring phase and an energy-releasing phase. Over the first three steps, glucose is converted into different forms and attached to two phosphate groups donated by two ATP molecules, resulting in an unstable sugar. In the next two stages, the unstable sugar splits into two sugar...
171.1K
Biosynthesis in Bacteria01:24

Biosynthesis in Bacteria

546
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
546
Amino Acid Biosynthetic Pathways01:29

Amino Acid Biosynthetic Pathways

1.0K
Amino acid biosynthesis is essential for cell growth, protein synthesis, and metabolic regulation. Cells generate essential and non-essential amino acids from metabolic intermediates to sustain vital biological functions. These intermediates originate from key metabolic pathways: glycolysis, the tricarboxylic acid (TCA) cycle, and the pentose phosphate pathway. Important precursors include α-ketoglutarate, pyruvate, oxaloacetate, phosphoenolpyruvate, and erythrose-4-phosphate, which...
1.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Endophytic microbiota and metabolites profile in gynoecious versus monoecious cucumbers.

BMC plant biology·2026
Same author

CellVizio<sup>®</sup> System for Mesothorax Lymphadenopathy Rapid on Site, 18G needle: Pros and Cons.

Journal of Cancer·2026
Same author

Asthma-free eosinophilic granulomatosis with polyangiitis presenting with Guillain-Barré syndrome-like symptoms: a case report.

Frontiers in medicine·2026
Same author

Macroscopic on-site evaluation based on core tissues length and weight during EBUS-TBNA using 22-gauge needles.

Chinese medical journal·2026
Same author

Exploration into the structural characteristics and functional properties of exopolysaccharide synthesized by Pseudoduganella armeniaca ZMN-3.

International journal of biological macromolecules·2026
Same author

Hemoadsorption combined with hemodialysis versus hemodialysis alone on mortality in end-stage kidney disease: a randomized, open-label, multicenter trial.

Nature communications·2026

相关实验视频

Updated: Jan 12, 2026

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
09:27

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues

Published on: February 17, 2017

10.7K

设计一种循环酶级联,以从马尔托德克斯基质中有效地进行三糖生物合成.

Qi Liu1, Yangyang Li1, Haidong Huang1

  • 1Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Science Center for Future Foods, Ministry of Education, Jiangnan University, Wuxi 214122, China.

Bioresource technology
|November 5, 2025
PubMed
概括

这项研究设计了酶,以改善三糖 (一种在食品和医药中使用的糖) 的生产. 一个新的酶级联使用马尔托德克斯实现了创纪录的84.31%的转化率,增强了生物合成三糖的生产.

关键词:
催化活动是催化活动.马尔托德克斯特林的水解.多酶级联是多种酶的级联.热稳定性 热稳定性这种树脂含有三糖.

更多相关视频

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
07:59

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

10.3K
In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast
06:53

In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

Published on: February 21, 2025

875

相关实验视频

Last Updated: Jan 12, 2026

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
09:27

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues

Published on: February 17, 2017

10.7K
A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
07:59

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

10.3K
In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast
06:53

In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

Published on: February 21, 2025

875

科学领域:

  • 生物技术是生物技术.
  • 酵素工程是什么? 酶工程是什么
  • 碳水化合物化学 碳水化合物化学

背景情况:

  • 糖是一种有价值的二糖体,由于其稳定性和蛋白质保护性质,在制药,食品和化品中具有应用.
  • 目前的工业三糖生产采用两步的酶过程 (maltooligosyltrehalose synthase和maltooligosyltrehalose trehalohydrolase),但由于转化率低和副产品积累而受到影响.
  • 低于最佳的酶性能和非可转换的短链马尔图糖的产生限制了现有的三糖生物合成途径的效率.

研究的目的:

  • 通过计算机辅助的酶工程来提高参与三糖生物合成的关键酶 (TreY和TreZ) 的热稳定性和催化效率.
  • 识别和整合一种新型酶,能够将积累的短链黄糖糖酸转化为可用于三糖路径的可用基质.
  • 开发一种高效,循环的多酶级联,用于从低成本基质中生产高效的生物合成三糖.

主要方法:

  • 计算机辅助的酶工程被用来修改来自Arthrobacter ramosus的maltooligosyltrehalose synthase (TreY) 和maltooligosyltrehalose trehalohydrolase (TreZ) 的酶.
  • 计算查发现了一个来自Corallococcus sp.的4-α-葡萄糖转移酶 (CSMalQ). 具有高效率的EGB在不成比例的短链马尔托酸糖中.
  • 通过整合工程Trey,TreZ和已识别的CSMalQ来构建一个循环多酶级联,使用马尔托德克斯作为主要基质.

主要成果:

  • 改造的TreZ突变T212P显示热稳定性增加了32.3%.
  • 改造的Trey突变M1显示了2.97倍的热稳定性改善和0.63倍的催化活性增加.
  • 综合循环多酶级联通过有效回收马尔托利戈糖,实现了84.31%的三糖转化率,这是迄今为止报告的最高水平.

结论:

  • 酶工程显著提高了三糖生物合成中的关键酶的性能.
  • 集成CSMalQ有效地解决了短链马尔托利高糖积的问题,提高了基质利用率.
  • 开发的循环多酶级联为工业化生物合成三糖生产提供了一个有前途和高效的战略.