Cilk1对于介质细胞纤维维维护和表皮细胞-介质细胞交叉在肠道形态发生过程中至关重要
Jieun Song1, Suyeon Je1, Bawool Lee2
1Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul, Republic of Korea; Brain Korea 21 (BK21) FOUR Program, Yonsei Education and Research Center for Biosystems, Yonsei University, Seoul, Republic of Korea.
Cellular and molecular gastroenterology and hepatology
|November 5, 2025
概括
产生相关激酶1 (Cilk1) 对于维护中酶体初级乳毛细胞至关重要,这对于肠发育至关重要. 它的干扰会损害 (Hh) 信号和半酶体聚类,导致发育缺陷.
科学领域:
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
- 胃肠病学 胃肠病学
背景情况:
- 肠道发育依赖于上皮细胞-介质细胞交叉声, (Hh) 信号对形态发生至关重要.
- 由表皮Hh信号驱动的介质细胞集群对于形成至关重要.
- 在这个过程中,中介细胞中初级乳头的作用尚未完全理解.
研究的目的:
- 研究 Ciliogenesis 相关激酶 1 (Cilk1) 在中酶体 ciliogenesis 中的作用.
- 确定Cilk1在肠发育过程中中介质细胞集群形成中的功能.
- 为了阐明Cilk1对初级乳毛维持和肠道介质细胞中Hh信号传递的影响.
主要方法:
- 使用Cilk1淘汰和特定组织删除的小鼠模型.
- 用有条件的Ift88敲击小鼠来评估初级毛的必要性.
- 应用免疫组织化学,qRT-PCR和RNA测序来分析乳毛存在,基因表达和信号通路.
主要成果:
- Cilk1对于维护胚胎14.5.5日以后的间酶体初级眼是必不可少的.
- Cilk1 缺失导致间细胞的毛囊损失,Hh 信号受损,缺陷的聚类和异常的毛囊形成.
- 转录组分析显示,BMP,Wnt和视网膜酸信号通路被破坏.
结论:
- Cilk1对于维护中酶体初级毛囊和支持肠形态发生是不可或缺的.
- Cilk1的干扰会影响Hh介导的细胞间细胞聚类,并影响BMP和Wnt的信号传递.
- 在肠道发育过程中,依赖于Cilk1的中细胞纤维生成对上皮细胞-中细胞通信至关重要.
相关概念视频
Microtubules in Signaling
2.1K
The primary cilium, made up of microtubules, acts as antennae on the cell surfaces for relaying external stimuli into the cells. These fine hair-like structures are present, generally one per cell. These are non-motile cilia in a 9+0 microtubules arrangement, where the central pair of microtubules are absent. The primary cilia arise from the basal body embedded in the cell membrane. Intraflagellar transport (IFT) carries requisite proteins from the cytoplasm to the cilium because the primary...
2.1K
Renewal of Intestinal Stem Cells
3.1K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
3.1K
Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.4K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.4K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.6K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.6K
Microvilli
8.8K
Microvilli are tiny finger-like projections found on the surface of certain cells. Their purpose is to increase the surface area of the cell's apical surface, resulting in more effective absorption or secretion of substances.
These microvilli are predominantly present in cells lining the small intestine, kidney tubules, and certain cells in the respiratory and reproductive systems. By significantly expanding the surface area of the cell membrane, microvilli enhance the cell's capacity...
These microvilli are predominantly present in cells lining the small intestine, kidney tubules, and certain cells in the respiratory and reproductive systems. By significantly expanding the surface area of the cell membrane, microvilli enhance the cell's capacity...
8.8K
Mechanism of Ciliary Motion
4.8K
The ciliary structures were first seen in 1647 by Antonie Leeuwenhoek while observing the protozoans. In lower organisms, these appendages are responsible for cell movement, while in higher organisms, these appendages help in the movement of the extracellular fluids within the body cavities.
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
4.8K


