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Bbe-miR-210通过准AmphiFos和AmphiJun来调节AMP-1-驱动的对LPS的先天免疫反应

Tao Fang¹, Luyao Gao¹, Jixu Zhang¹

¹Laboratory for Comparative Genomics and Bioinformatics & Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing, 210046, China.

Developmental and comparative immunology

|November 5, 2025

概括

我们发现两种激活蛋白1 (AP-1) 的子单元,AmphiFos和AmphiJun,在amphioxus中,这对天生的免疫非常重要. 一个微RNA,bbe-miR-210,调节这些AP-1子单元以平衡免疫反应.

科学领域:

进化生物学是进化的生物学.
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

激活蛋白1 (AP-1) 转录因子对于哺乳动物的细胞过程至关重要.
之前,AP-1在脊椎动物起源的关键模型 - - 两动物中的作用是未知的.
安菲奥克斯 (Amphioxus) 提供了关于免疫和发育进化的见解.

研究的目的:

发现和功能性地描述两类 (Branchiostoma belcheri tsingtauense) 中的新型AP-1子单元.
研究这些AP-1亚单元在先天免疫反应中的作用.
为了探索AP-1活动的调节机制在这个基底带.

主要方法:

鉴定和描述了两种AP-1同类物种:AmphiFos和AmphiJun.
评估了AmphiFos和AmphiJun在对脂多糖 (LPS) 的天生的免疫反应中的参与.
研究了AmphiFos/AmphiJun异构体的DNA结合特异性和转录激活能力.
确定了bbe-miR-210作为一个针对AmphiFos和AmphiJun的微RNA.

主要成果:

AmphiFos和AmphiJun被确定为amphioxus中的新AP-1子单元.
这两种子单元都在对LPS的天生的免疫反应中发挥作用.
这种AmphiFos/AmphiJun异构体特别与AP-1动机结合,并增强细胞因子的表达.
发现bbe-miR-210直接向并抑制AmphiFos和AmphiJun的表达,调节免疫反应.

结论:

这项研究揭示了AP-1介导的免疫调节在基底带动物的进化保存.
发现了一种涉及bbe-miR-210微调amphioxus免疫中的AP-1活性的新机制.
这些发现增强了我们对脊椎动物免疫系统起源的理解.

关键词:

激活蛋白质 1 激活蛋白质 1 阿姆菲奥克苏斯 (Amphioxus) 是一个类动物.进化进化进化进化进化进化进化这是天生的免疫力.在 miR-21010 里面,

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