氨和水基银化物对牙原蛋白基质的长期影响
Merve Uctasli1, Roda Seseogullari-Dirihan2, Thiago Henrique Scarabello Stape3
1Department of Restorative Dentistry and Cariology, Adhesive Dentistry Research Group, Institute of Dentistry, University of Turku, Turku, Finland.
概括
银化物治疗可能会减缓牙原蛋白的降解. 然而,添加酸会增加蛋白酶活性,损害牙.
科学领域:
- 生物材料科学 生物材料科学
- 牙科研究 牙科研究
- 生物化学 生物化学
背景情况:
- 牙原蛋白矩阵降解是恢复性牙科的一个重要问题.
- 化银 (SF) 用于皮管理,但其对牙的长期影响尚未完全理解.
- 了解SF,化 (KI) 和牙的有机矩阵之间的相互作用对于优化治疗结果至关重要.
研究的目的:
- 研究以氨和水为基础的化银 (SF) 处理对牙原蛋白降解的长期影响.
- 评估化 (KI) 与SF对牙基质结构完整性的影响.
主要方法:
- 无矿化牙束被用SF (以氨或水为基础),SF + KI或单独使用KI处理,并与未经处理的对照组进行比较.
- 在人工唾液中化6个月后,评估了干质量,弹性模量和酶活性的变化.
- 分析了原降解标记物 (ICTP,CTX,素,总蛋白),并进行了SEM和现场生殖图.
主要成果:
- SF治疗降低了整体酶活性,但随着时间的推移增加了溶解的原.
- 添加KI显著加剧了干性质,弹性模量,基和总蛋白质的损失.
- SF治疗显示有潜力减轻原体降解,而KI则加剧了这种情况.
结论:
- 以氨和水为基础的化银处理可能会提供长期保护,防止牙原蛋白降解.
- 化与SF相结合,可以增加内源蛋白酶活性,从而对牙的结构完整性产生负面影响.
- 需要仔细考虑SF和KI的组合,以避免损害牙的有机基质.
相关概念视频
01:19Diazonium Group Substitution: –OH and –H
Nitrous acid, a weak acid, is prepared in situ via the reaction of sodium nitrite with a strong acid under cold conditions. This nitrous acid prepared in situ reacts with primary arylamines to form arenediazonium salts. Such reactions are known as diazotization reactions. As shown in Figure 1, the formation of arenediazonium salts begins with the decomposition of nitrous acid in an acidic solution to give nitrosonium ions.
01:26EDTA: Auxiliary Complexing Reagents
EDTA titrations are usually carried out in highly basic conditions, where the fully deprotonated form of EDTA, Y4−, actively complexes with the free metal ions in the solution. Several metal ions precipitate as hydrous oxide (hydroxides, oxides, or oxyhydroxides) under these conditions, lowering the concentration of free metal ions in the solution. For this reason, auxiliary complexing agents or ligands such as ammonia, tartrate, citrate, or triethanolamine are used in EDTA titrations to...