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相关概念视频

Subcellular Fractionation01:32

Subcellular Fractionation

8.5K
The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
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Cell Specific Gene Expression01:58

Cell Specific Gene Expression

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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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Cell Specific Gene Expression01:58

Cell Specific Gene Expression

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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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相关实验视频

Updated: Jan 12, 2026

Mining Spatial Transcriptomics Datasets using DeepSpaceDB
10:16

Mining Spatial Transcriptomics Datasets using DeepSpaceDB

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细胞SP使得模块发现和可视化为亚细胞空间转录学数据的可视化.

Bhavay Aggarwal1, Saurabh Sinha2,3

  • 1The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.

Communications biology
|November 5, 2025
PubMed
概括
此摘要是机器生成的。

细胞SP是一个新的计算框架,可以识别和可视化亚细胞mRNA模式. 这种工具揭示了基因细胞模块,为各种生物过程提供了功能性见解,例如大脑发育和疾病.

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Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
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Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

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Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
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相关实验视频

Last Updated: Jan 12, 2026

Mining Spatial Transcriptomics Datasets using DeepSpaceDB
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Mining Spatial Transcriptomics Datasets using DeepSpaceDB

Published on: September 5, 2025

632
Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
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Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

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Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
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科学领域:

  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.
  • 基因组学就是基因组学.

背景情况:

  • 空间转录学允许研究细胞内的mRNA分布,这对细胞功能至关重要.
  • 现有的工具缺乏识别和解释功能相关的亚细胞转录模式的能力.

研究的目的:

  • 介绍CellSP,一个用于识别,可视化和表征一致的亚细胞空间mRNA模式的计算框架.
  • 引入"基因细胞模块",代表具有协调的亚细胞转录分布的基因组.

主要方法:

  • 开发CellSP,一个计算框架.
  • 使用"基因细胞模块"来分析协调的转录分布.
  • 发现的模块的功能丰富分析.

主要成果:

  • 细胞SP可靠地识别不同组织和技术的功能重要模块.
  • 在小鼠大脑中发现与髓化,轴突发生和突触形成相关的亚细胞空间现象.
  • 在癌中识别免疫反应模块和在阿尔茨海默病模型中的髓化模块.

结论:

  • 细胞SP提供了一种强大的方法来剖析亚细胞转录本地化.
  • 该框架提供了对生物过程和疾病状态的功能性见解.
  • 细胞SP有助于发现新的空间转录体现象.