由单细胞引起的机械诱导模式域的形成.
Wenxuan Du1,2, Jingyi Zhu2,3, Yufei Wu2,4
1Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA.
Nature materials
|November 5, 2025
概括
人类单细胞在柔软的水凝上形成多细胞域,这种独特的行为通过改变细胞粘附和迁移来增强它们的生存. 这一发现揭示了组织硬反应中的集体细胞动态.
科学领域:
- 生物物理学的生物物理.
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
背景情况:
- 细胞表型和功能受到矩阵刚度和机械信号的显著影响.
- 组织硬度对单细胞行为的影响和集体细胞行为的出现仍然未得到充分研究.
研究的目的:
- 研究组织度如何影响人体初级单细胞的行为.
- 了解单细胞中集体细胞行为形成背后的机制.
主要方法:
- 利用具有生理性的软原蛋白涂层水凝培养人类初级单细胞.
- 采用计算建模,包括Cahn-Hilliard方程和图灵机制,以模拟和理解域形成.
- 实验验证的计算模型预测关于细胞播种密度,化学反应和随机迁移.
主要成果:
- 人类单细胞独特地在软水凝上形成动态,规律,可逆,多细胞域.
- 细胞间粘附是由β2整合素-ICAM-1复合体启动的,而可溶性抑制因子保持域稳定性.
- 域形成增强了单细胞的存活能力,尽管阻碍了细胞的能力.
结论:
- 细胞可以通过利用机械感知,短距离相互作用和远距离信号来产生复杂的相位.
- 单细胞的集体行为和域形成是由局部激活和全球抑制机制的平衡驱动的.
- 这项研究揭示了由矩阵刚性和集体行为介导的单细胞的新生存策略.
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