使用酶生物传感器测试条进行唾液酸盐的非侵入性和用户友好的视觉检测,用于慢性病查
Farah Gull1, Seham J F Alanazi2, Sehrish Bilal3
1Interdisciplinary Research Center in Biomedical Materials (IRCBM), COMSATS University Islamabad, Lahore Campus, 1.5 Km Defense Road, off Raiwind Road, Lahore, Punjab, 54000, Pakistan.
Analytical and bioanalytical chemistry
|November 5, 2025
概括
这项研究引入了一种新的纸质测试条来检测唾液酸盐,为慢性病 (CKD) 查提供了一个非侵入性的替代方案. 颜色测量平台为酸盐水平评估提供了一种实用且以患者为中心的方法.
科学领域:
- 生物医学工程 生物医学工程
- 分析化学 分析化学
- 临床诊断 临床诊断 临床诊断
背景情况:
- 慢性病 (CKD) 查传统上依赖于侵入性血液检测.
- 唾液酸盐是一种新兴的,可访问的CKD生物标志物.
- 需要非侵入性,患者友好的诊断工具.
研究的目的:
- 开发和验证一种纸质色度生物传感器,用于唾液酸盐检测.
- 建立唾液作为非侵入性CKD查的实用媒介.
- 提供以患者为中心的替代血液酸盐测定.
主要方法:
- 一个基于纸张的平台,集成了唾液收集条和水凝传感模块.
- 在酸 (SA) 水凝中封装酸酶 (ALP) 酶.
- 通过ALP抑制对酸盐的色度检测,使用RGB图像分析量化.
主要成果:
- 生物传感器显示线性检测范围为0.15-10毫米,检测极限 (LOD) 为0.12毫米.
- RGB分析显示了与光谱光度学结果的强烈相关性,验证了该方法的可靠性.
- 观察到高选择性,灵敏性,可再生性和稳定性,人工唾液样本的恢复率为92-99%.
结论:
- 开发的基于纸张的生物传感器提供了一种快速,非侵入性和可靠的唾液酸盐量化方法.
- 这项技术为CKD查和个性化治疗监测提供了可行的替代方案.
- 以患者为中心的方法提高了诊断策略的可访问性和合规性.
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