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一种用于向抑制真菌生长的新型酶方法

Angelina Ivanova¹, Valeriia Buzova^2,3

¹SkyLab AG, Superlab Suisse Epalinges SA, Route de la Corniche 6, Epalinges, Lausanne, 1066, Switzerland. ivanova@skylaboratory.ch.

BMC microbiology

|November 5, 2025

概括

基因酶和基因酶酶的组合有效地抑制了毛菌种,这是头皮的主要原因. 这种有针对性的方法显示出显著的真菌减少,并改善了头皮微生物群的多样性,没有广泛的抗菌作用.

科学领域:

皮肤病学和真菌学
生物化学和酶技术生物化学和酶技术
微生物组研究微生物组研究

背景情况:

头发是一种多因素头皮疾病,与障碍功能和微生物不平衡有关.
马拉塞西亚物种 (M. furfur,M. restricta,M. globosa) 的过度殖民是主要的致病机制.
传统的抗真菌治疗可以引起刺激,并对头皮微生物组产生负面影响.

研究的目的:

为了评估基因酶和基因酶酶组合对头皮马拉塞西亚物种的疗效.
评估这种酶方法对头皮微生物群的安全性和影响.
将新型酶治疗与标准抗真菌剂进行比较.

主要方法:

在体外抗真菌活性测试基因酶和基因酶对马拉西亚菌种的测试.
与传统的抗真菌药物 (climbazole,piroctone olamine等) 的比较 ) 的情况.
使用纤维细胞细胞系进行细胞毒性评估 (MTT检测,光显微镜).
临床研究 (n=18) 通过qPCR评估头皮M.furfur和M.restricta的DNA/RNA水平.
基因组学分析以评估对细菌组成和多样性的影响.

主要成果:

结合酸酶和酸酶 (0.25%) 实现了98.38%的Malassezia spp.的抑制,完全抑制0.5%.
临床研究显示,在头皮治疗3小时后,M.furfur和M.restrictaDNA (2.4倍,1.9倍) 和RNA (2.4倍,4.6倍) 的显著减少.
酶治疗增加了头皮细菌的α多样性,并使操作分类单元 (OTU) 的数量翻了一番.
只有在度≥7.6%时才观察到细胞毒性,远远超过有效的抗真菌剂量.

结论:

酸酶-酸酶组合提供了一种有针对性和有效的策略,用于控制头皮马拉西亚物种.
这种酶方法显示出有利的安全性,并促进了头皮微生物组的健康.
酸酶和酸酶是用于皮管理的传统抗真菌药物的有希望的替代品.

关键词:

马拉塞西亚 spp.抗真菌剂是一种抗真菌剂.基因酸酶的使用方法基托桑酶是一种基托桑酶.气候变化造成的危害皮肤的治疗方法二醇烯酸盐是一种二醇.头皮上的微生物组.

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