来自伊朗的泌尿病原性大肠杆菌分离物中酸黄素耐药性机制
Shayesteh Novin1, Seyedeh Elham Rezatofighi2, Mohammad Roayaei Ardakani3
1Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Annals of clinical microbiology and antimicrobials
|November 5, 2025
概括
泌尿病原性大肠杆菌 (UPEC) 中的酸黄素耐药性是由染色体基因nfsA和nfsB的突变驱动的. 这些耐药的UPEC菌株表现出多种药物耐药性和具有毒性因子,需要进一步的流行病学研究.
科学领域:
- 微生物学 微生物学
- 遗传学 遗传学 是一个
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 尼托福兰是治疗无并发性尿路感染 (UTI) 的关键抗生素.
- 泌尿病原性大肠杆菌 (UPEC) 的新兴耐药性构成了重大的临床挑战.
- 了解这种耐药性的遗传基础对于有效的治疗策略至关重要.
研究的目的:
- 为了研究耐素抗性UPEC分离物的遗传多样性.
- 描述与UPEC中耐黄因耐药性相关的机制和遗传特征.
主要方法:
- 一项横截面研究分析了235个UPEC分离物以检测耐素的耐药性.
- 聚合酶链反应 (PCR) 用于检测染色体 (nfsA,nfsB,ribE) 和等离子体 (oqxA,oqxB) 基因.
- 基因测序确定了nfsA,nfsB和ribE的变异;表型和基因型特征也被评估.
主要成果:
- 确定了六个 (2.55%) 耐尼托福兰因耐药的UPEC分离体.
- 检测到nfsA和nfsB中的有害突变是可能的耐药性驱动因素;没有发现ribE突变和等离子体基因qxA/oqxB.
- 所有耐药分离物都是多抗药性 (MDR),并携带各种毒性相关基因 (VAG) 和致病性岛屿 (PAI).
结论:
- 染色体nfsA和/或nfsB基因中的有害突变是研究UPEC隔离物中尼托福兰因耐药性的主要驱动因素.
- 在 ribE 和通过 oqxAB 的等离子体介导耐药性的突变似乎起到较小的作用.
- 抗性和毒性因子在这些分离物中同时出现,强调需要更广泛的流行病学评估.
相关概念视频
01:30Development of Antibiotic Resistance
1.2K
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
1.2K
01:30Urinary Tract Infection III: Diagnostic Studies and Interprofessional Care
239
A healthcare provider can diagnose a urinary tract infection (UTI) through several methods:Medical History and Symptoms: The provider will take a detailed medical history and ask about symptoms such as frequent urination, burning sensation during urination, and lower abdominal pain.Urinalysis: A clean-catch urine sample is collected in a sterile container and tested for the presence of bacteria, white blood cells (leukocytes), nitrites, blood, and protein. The presence of leukocytes and...
239
01:26Urine Studies II: Urine Culture and Sensitivity Test
798
A urine culture and sensitivity test is a diagnostic procedure used to identify urinary tract bacterial infections and determine the most effective antibiotics for treatment. This test is generally preferred when a patient shows manifestations of a urinary tract infection, such as frequent or painful urination, cloudy or foul-smelling urine, or lower abdominal pain.Purpose of the TestThe primary goals of a urine culture and sensitivity test are to:Determine the specific bacteria causing the...
798
00:57Antibiotic Selection
59.3K
Overview
59.3K
01:23Stringent Response in E. coli
277
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
277
01:27Combined Effects of Drugs: Synergism
6.7K
Synergism is a useful mechanism where combining two or more drugs is more effective than each constituent used alone. Such combinations are also called supra-additive interactions. The drugs collectively enhance the final therapeutic effect by acting on different targets. Another advantage is that the low dose of each constituent drug is sufficient to achieve the desired effect. This helps reduce the duration of therapy and lower the adverse effects of these drugs.
Such synergistic combinations...
Such synergistic combinations...
6.7K