在心肌组织中进行浸泡式清除和自流光火
Victoria E Sturgess1, Nadia K Korovesis1, Domingo E Uceda2
1Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
Microcirculation (New York, N.Y. : 1994)
|November 6, 2025
概括
这项研究优化了基于沉浸的协议,用于使用番茄莱克和CUBIC清除在心肌组织中的3D微血管成像. 该方法增强了在大型动物模型中冠状动脉微血管结构的实际可视化.
科学领域:
- 生物医学工程 生物医学工程
- 光学显微镜的使用方法
- 历史学 历史学 历史学
背景情况:
- 微血管网络的3D可视化正在通过新的光学显微镜和组织制备技术取得进展.
- 组织清除通过增强光线透来改善成像深度.
- 对较大的组织样本而言,浸泡式方法比 perfusion 方法具有实际优势.
研究的目的:
- 为微血管标记和肌肉组织组织的组织清除提供一个优化的沉浸式协议.
- 为了证明番茄莱克和CUBIC清除对于深层组织成像的有效性.
- 评估优化脱脂和火阶段对图像质量的影响.
主要方法:
- 开发了一种浸泡方案,使用番茄莱克和CUBIC清除用于老鼠和猪心肌组织.
- 优化脱脂和火步骤,用于增强成像.
- 使用信号噪声比 (SNR) 和平均z-slice强度评估图像质量.
主要成果:
- 在CUBIC Reagent I中24小时化后,获得了最佳的图像质量.
- 一些灭剂 (TrueBlack,Sudan Black B) 显示了对图像深度降低的趋势.
- 与猪组织相比,大鼠心肌组织的SNR值较高.
结论:
- 开发的协议提供了一种可靠的方法来优化基于浸泡的心肌组织清除.
- 这项技术可为冠状动脉微血管结构提供详细的3D成像.
- 未来的研究将利用这个协议来比较不同疾病状态中的微血管解剖学.
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