超高通量粘弹性挤压用于机械孔和高效的细胞间传输
Rashin Mohammadi1, Irma Sakic2,3, Ankit Jain1
1Institute for Chemical and Bioengineering, ETH Zürich, Zürich, 8093, Switzerland.
Small (Weinheim an der Bergstrasse, Germany)
|November 6, 2025
概括
一种新的粘弹性挤压方法增强了生物分子向细胞的输送,用于先进的治疗方法. 这种技术提供了高效率和细胞活力,克服了病毒载体和电穿孔等传统方法的局限性.
科学领域:
- 生物技术是生物技术.
- 细胞工程 细胞工程
- 微流体学 微流体学
背景情况:
- 基于细胞的疗法需要有效的细胞内传递生物分子.
- 现有的方法 (病毒载体,电穿孔) 面临诸如细胞毒性和低吞吐量等挑战.
研究的目的:
- 引入一种新方法,快速,无接触地将货物送入单元.
- 为了克服当前细胞输送技术的局限性.
主要方法:
- 利用粘弹性微流体来创建细胞变形的"虚拟通道".
- 控制细胞/货物流和流的流量,以调节通道宽度.
- 通过机械穿孔利用弹性力来实现有效载荷的内部化.
主要成果:
- 证明了mRNA,等离子体DNA和CRISPR-Cas9 RNP复合体的成功输送.
- 与传统的分孔方法相比,实现了更高的交付效率.
- 保持高细胞活力,每分钟处理多达2000万个细胞.
结论:
- 粘弹性挤压是一种强大,高效和温和的细胞工程方法.
- 这个平台为开发先进的基于细胞的疗法提供了显著的优势.
- 该技术显示出在基因工程和药物输送方面广泛应用的前景.
相关概念视频
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