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循环的microRNA-222和microRNA-146b:一种潜在的区分工具,可以区分良性和恶性甲状腺结节

Ramy Samir Assaad¹, Wafaa Saad Ragab¹, Rania El Tayfi²

¹Chemical Pathology Department, Medical Research Institute - Alexandria University, Alexandria, Egypt.

Scandinavian journal of clinical and laboratory investigation

|November 6, 2025

概括

血miRNA-222和miRNA-146b在恶性甲状腺结核中的表达增加,与良性结核相比. 这些microRNA可能会改善目前甲状腺结节的诊断方法.

科学领域:

内分泌学在内分泌学.
分子生物学分子生物学
在瘤学瘤学.

背景情况:

甲状腺结节很常见,大多数是良性的,但区分恶性结节至关重要.
目前的诊断方法,如超声波和精细针吸入细胞学 (FNAC) 有局限性,往往需要手术来确定确诊.
越来越需要分子工具来准确区分良性和恶性甲状腺结节.

研究的目的:

研究血微RNA-222 (miRNA-222) 和微RNA-146b (miRNA-146b) 作为生物标记物的潜力,以区分良性和恶性甲状腺结节.
评估这些microRNAs的诊断性能,单独和与现有方法结合使用.

主要方法:

一项病例控制研究涉及46名患有甲状腺结节的成人患者 (23名良性,23名恶性),经手术后遗传病理学证实.
在手术前收集了血样本,用于RNA提取和定量实时PCR (RT-qPCR) 分析miRNA-222,miRNA-146b和参考miRNA-16.
来自40名健康个体的血作为对照.

主要成果:

在患有恶性结节的患者中,与患有良性结节的患者相比,观察到miRNA-222和miRNA-146b的显著更高的血表达 (p <0.001两者).
超过尤登指数切线的miRNA-222或miRNA-146b血水平升高显示良性和恶性群体之间存在显著差异 (p < 0.001).
血miRNA-222表达的增加与恶性结核中的血管入侵相关. 将miRNA水平与超声波和FNAC相结合,提高了诊断准确度 (ROC的AUC).

结论:

血miRNA-222和miRNA-146b显示出作为区分良性和恶性甲状腺结节的非侵入性生物标志物的前景.
这些微RNA可以作为当前诊断工作的宝贵补充,可能减少不必要的手术.

关键词:

甲状腺癌是什么?甲状腺癌是什么?这就是miRNA-146bb.微型RNA-2222222的使用情况.这是一个微型RNA.甲状腺结节甲状腺结节

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...