二色的转录因子LbYABBY1通过抑制LbSAD2通路来提高盐敏感性
Boqing Zhao1, Yaqiong Sui1, Baoshan Wang1
1Shandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Ji'nan, 250014, China.
The Plant journal : for cell and molecular biology
|November 6, 2025
概括
YABBY转录因子LbYAB1负面调节了Limonium bicolor中的盐腺发育,影响了盐度耐受性. 这一发现为设计耐盐作物提供了潜力.
科学领域:
- 植物生物学 植物生物学
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
背景情况:
- 双色球,一个recretohalophyte,拥有盐腺,以适应盐水土壤.
- YABBY (YAB) 转录因子参与植物发育和应激反应.
- 以前的研究表明,L. bicolor. 的盐腺细胞中LbYAB1的高表达.
研究的目的:
- 为了确认LbYAB1在L.双色球中的表达模式.
- 阐明 LbYAB1 在盐腺发育和盐度耐受性中的作用.
- 确定涉及LbYAB1.1.的相互作用蛋白和调节通路.
主要方法:
- 在基因表达确认的RNA in situ杂交和GUS染色.
- 产生 LbYAB1 过度表达和敲除工厂.
- 酵母两杂交,分裂-露西法酶补充和GST拉下测试以研究蛋白质相互作用.
主要成果:
- LbYAB1在盐腺和叶子中表达,表达由6-氨酸氨酸诱导.
- 过度表达LbYAB1抑制了盐腺发育和降低了盐分耐受性,而敲击则增强了这些特征.
- LbYAB1与LbKNAT7相互作用,增强抑制LbSAD2的作用,LbSAD2是盐腺发育的积极调节者.
结论:
- 在 L. bicolor. 中,LbYAB1 作为盐腺发育的负调节剂.
- LbYAB1-LbKNAT7复合体负面调节LbSAD2,控制盐腺发育和盐度耐受性.
- 这项研究提供了对盐度耐受性机制和改善作物的潜在策略的见解.
相关概念视频
Responses to Salt Stress
14.4K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
14.4K
Transcription Attenuation in Prokaryotes
18.1K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
18.1K
Transcription
154.9K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
154.9K
Stringent Response in E. coli
277
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
277
Adaptations that Reduce Water Loss
27.9K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
27.9K
Transcriptional Regulation: Riboswitches
546
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
546


