作为β-葡萄糖的新来源的Pichia kudriavzevii酵母细胞壁:提取,表征和益生菌功能
Buse Dengiz1,2, Zehranur Yuksekdag3, Seniha Selcen Babaoglu-Aydas4
1Graduate School of Natural and Applied Science, Gazi University, Ankara, Türkiye.
Folia microbiologica
|November 6, 2025
概括
这项研究优化了酵母β-葡萄糖的提取,产生了强大的益生菌. 这种Pichia kudriavzevii M13菌株是Pichia kudriavzevii M13菌株.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 食品科学 食品科学 食品科学
背景情况:
- β-葡萄糖是一种具有显著健康益处的多糖,包括益生菌和抗氧化物质.
- 酵母细胞壁是β-葡萄糖的丰富来源,但提取效率和功能特征对于应用至关重要.
- 从特定的酵母菌株中优化提取是最大限度地提高产量和理想性质的关键.
研究的目的:
- 为了优化从Pichia kudriavzevii M13.中提取β-葡萄糖.
- 描述提取的β-葡萄糖的结构和功能性质.
- 评估用于功能性食品的β-葡萄糖的益生菌潜力和胃肠道稳定性.
主要方法:
- 对五种酵母菌株 (P. kudriavzevii M5,M10,M13,M16,M57) 的β-葡萄糖含量进行选.
- 提取条件的优化,包括注射,自解,热水,超声波和蛋白酶处理.
- 通过Ligilactobacillus plantarum GD2,Bifidobacterium bifidum A12和Saccharomyces cerevisiae BD1.1进行β-葡萄糖发酵性的评估.
- 评估益生菌活动 (LGP,BGP,MGP) 和抗氧化能力 (DPPH测定).
- 在模拟的胃和胆汁条件下测试胃肠道稳定性.
主要成果:
- Pichia kudriavzevii M13产生了最高的β-葡萄糖含量 (87.8%),并被选择进行进一步研究.
- 提取的β-葡萄糖 (β-gluM13) 显示出显著的乳酸菌,双菌和酵母促进生长 (LGP,BGP,MGP) 活动.
- β-gluM13表现出适度的抗氧化活性,与酸可比,并在模拟的胃和胆汁液中表现出极好的稳定性.
- 作为唯一的碳来源,β-gluM13的存在增加了有益肠道细菌的活力.
结论:
- 从Pichia kudriavzevii M13中进行优化提取,可以获得高纯度的β-葡萄糖,具有显著的益生菌潜力.
- β-gluM13对关键的肠道微生物具有有益的增长促进作用,并表现出良好的抗氧化特性.
- 经过证明的胃肠道稳定性和功能性质将β-gluM13定位为功能性食品和肠道健康应用中的有希望的成分.
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