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基于生物信息学分析和实验验证的潜在生物标志物和低活性膀的治疗点的识别

Chen Chen^1,2, Zhuojing Hu³, Yunbo Ma¹

¹Department of Urology, Liaocheng People's Hospital, Liaocheng, Shandong, China.

|November 6, 2025

概括

这项研究确定了像CLEC4E,CSF3R,CXCR2,FPR2和IDO1这样的关键基因,作为低活性膀 (UAB) 的潜在诊断生物标志物. 这些发现可能会导致UAB管理的新治疗策略.

科学领域:

泌尿器科泌尿器科泌尿器科泌尿器科
生物信息学是一种生物信息学.
分子生物学分子生物学

背景情况:

低活性膀 (UAB) 显著影响患者的生活质量.
了解UAB的分子机制对于有效治疗至关重要.
目前的管理策略需要通过新的治疗目标进行改进.

研究的目的:

确定UAB的关键枢纽基因和潜在的治疗点.
阐明与UAB相关的基因表达特征.
验证已识别的枢纽基因的诊断潜力.

主要方法:

对GEO数据集进行生物信息学分析 (GSE122060,GSE100219,GSE28242).
鉴定差异表达基因 (DEGs) 和构建蛋白质-蛋白质相互作用 (PPI) 网络.
功能丰富分析,枢纽基因查,ROC曲线分析和qRT-PCR验证.

主要成果:

确定了85个DEG;功能丰富突出显示了中性粒细胞迁移和化学反应.
在PPI网络中发现了12个关键基因;CLEC4E,CSF3R,CXCR2,FPR2和IDO1显著上调.
六个枢纽基因表现出高诊断潜力 (AUC>0.76);确定了调节这些基因的TF和miRNA.

结论:

作为UAB的诊断生物标志物,CLEC4E,CSF3R,CXCR2,FPR2和IDO1显示出有希望的结果.
已识别的TF和miRNA为UAB提供了新的药物发现和治疗干预的潜力.
该研究提供了对UAB进展背后的分子机制的见解.

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