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通过废水监测检测和量化关键牙科病原体

Olivia N Birch¹, Sang C Par¹, Justin C Greaves¹

¹Department of Environmental and Occupational Health, School of Public Health Indiana University-Bloomington.

|November 6, 2025

概括

基于废水的流行病学成功地监测了废水中的常见口腔细菌Streptococcus mutans和Porphyromonas gingivalis. 这表明废水监测有潜力追踪牙科病原体并了解它们的传播途径.

科学领域:

环境微生物学环境微生物学
公共卫生监督是对公共卫生的监督.
口腔微生物学口腔微生物学

背景情况:

基于废水的流行病学 (WBE) 已建立用于病毒病原体跟踪.
对于常见的口腔细菌病原体,WBE的应用仍然未被探索.
菌突变菌和 Porphyromonas gingivalis 是主要的病原体,导致牙和牙炎等流行牙疾病.

研究的目的:

调查废水中S. mutans和P. gingivalis的存在和流行情况.
确定使用WBE用于监测口腔细菌病原体的可行性.
评估口腔细菌通过废水传播的潜在传播途径.

主要方法:

在24个月内 (2023年6月 - 2025年5月) 收集每周未经处理的废水样本.
集中样本,提取的DNA,以及量化的S. mutans和P. gingivalis核酸.
在未经处理的,初级和最终的废水中分析了细菌的存在.

主要成果:

在89%的样本中检测到S. mutans;在58%的样本中检测到P. gingivalis.
平均度:S. mutans的4.57 log10基因组副本/L和P. gingivalis的3.03 log10基因组副本/L.
口腔细菌在废水中被发现,但度低于未经处理的废水.

结论:

废水监测是跟踪口腔细菌的一种可行的方法.
高度表明S. mutans可能通过水传播.
WBE提供了一种用于监测口腔细菌病原体和公共牙健康的新方法.

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