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把拼图拼在一起:在单细胞分辨率下对植物的分析

Nora Damaris Pasquali Medici de Biron¹, Sara Farrona¹

¹School of Biological and Chemical Sciences, College of Science and Engineering, University of Galway, H91 TK33, Galway, Ireland; Agriculture, Food Systems & BioEconomy Research Centre, Ryan Institute, University of Galway, H91 TK33, Galway, Ireland.

Current opinion in plant biology

|November 6, 2025

概括

单细胞和单核米技术为植物生物学和作物改进提供了强大的洞察力. 本综述指导研究人员在农业创新中应用这些先进方法.

科学领域:

植物生物学植物生物学
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

单细胞和单核米技术正在快速推进植物研究.
RNA测序和染色体可访问性分析为植物发育,细胞身份和应激反应提供了深入的见解.
转录和染色质可访问性数据的整合将监管元素与基因表达联系起来.

研究的目的:

为目前的方法,生物信息学工具和植物单细胞/单核化学的应用提供实用指南.
为实施这些技术所带来的机遇和挑战提供一个清晰的视角.
为了使有关植物研究协议选择的知情决策.

主要方法:

对单细胞RNA测序 (scRNA-seq) 的审查以及对植物中转移酶可访问的染色质测序 (ATAC-seq) 的测定.
综合生物信息工具用于分析多原子数据.
突出新兴的多元经济战略.

主要成果:

这些技术为植物发育,细胞身份和应激反应提供了前所未有的洞察力.
数据的整合将监管元素与多种植物组织的基因表达联系起来.
应用涵盖了各种植物物种和研究领域.

结论:

在植物中扩大单细胞/单核基因的使用将促进基础生物学.
这些技术为改善作物和农业创新产生可操作的见解.
促进将单细胞发现转化为实际的农业应用.

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