来自Trichoderma longibrachiatum的西兰酶在模拟的人类胃肠道条件下从小麦西兰中产生西洛利高糖:一种新的益生菌策略
Caroline H Best1, Yunyao Qu1, Kelly M Tinker1
1Department of Research and Development, BIO-CAT, Inc., 9117 Three Notch Rd, Troy, VA 22974, USA.
Food chemistry
|November 6, 2025
概括
这项研究表明,Trichoderma longibrachiatum xylanase (TLX) 能够有效地分解小麦纤维,增加益生菌氧化糖 (XOS) 和降低粘度. TLX显示了改善植物性饮食和肠道健康的潜力.
科学领域:
- 酶学 是一种酶学.
- 营养科学 营养科学
- 微生物学 微生物学
背景情况:
- 兰是复杂的植物纤维,对人类健康至关重要.
- 氨酶,如Trichoderma longibrachiatum中的TLX,可以分解氨酸.
- 优化纤维消化是肠道健康的关键,特别是随着植物性饮食的增加.
研究的目的:
- 为了评估TLX在模拟人体消化下水解富含西兰的食物中的有效性.
- 评估TLX产生 prebiotic xylooligosaccharides (XOS) 和降低消化器粘度的能力.
- 为了确定TLX对不同植物性材料的基质特异性.
主要方法:
- 利用INFOGEST在体外静态消化模型模拟人类胃和肠道状况.
- 使用高性能液体染色学 (HPLC),粘度测试和液体染色学-质谱学 (LC-MS) 评估TLX活性.
- 在富含西兰的基质上测试了TLX,包括基于小麦和大米的食品.
主要成果:
- TLX显著增加了基于小麦的基质的益生菌XOS (DP2-6) 和糖生产.
- TLX显著降低了基于小麦的消化剂的粘度.
- TLX证明了基质特异性,从大米中的大米饼干中释放的XOS (DP2-10) 是大米中的350倍.
结论:
- 在植物性饮食中,TLX有效地提高了纤维消化能力和益生菌XOS的可用性.
- 通过优化纤维水解,TLX显示出改善肠道微生物群健康的前景.
- 这些发现支持TLX在食品应用中的使用,以利用食纤维的好处.
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