相关实验视频

Updated: Aug 3, 2026

Detection of Protein Ubiquitination

Detection of Protein Ubiquitination

Published on: August 19, 2009

使用ThUBD涂层的高密度96孔板进行特定,快速,精确和高效的蛋白质无化检测

Hui Liu¹, Huanxiang Chen², Heng Li³

¹State Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Beijing, 102206, China; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, College of Chemistry & Materials Science, Hebei University, Baoding, Hebei, 071002, China.

|November 6, 2025

概括

研究人员开发了一个高通量平台,使用Tandem Hybrid Ubiquitin Binding Domain (ThUBD) 蛋白质进行敏感和特定的检测. 这一进步克服了现有工具的局限性,使所有无处不在链类型的无偏量化成为可能.

科学领域:

生物化学生物化学
分子生物学分子生物学
细胞信号传输细胞信号传输

背景情况:

蛋白质无处不在对于细胞信号传递至关重要.
无处不在的失调与各种疾病有关.
目前用于检测无处不在的方法在亲和力和链接偏差方面存在局限性,这阻碍了高通量分析.

研究的目的:

开发一个高通量,敏感和特定的平台来识别和量化无处不在的蛋白质.
克服现有的无处不在检测工具的局限性.
为了使所有无处不在链类型的无偏捕获.

主要方法:

采用了一种无偏的,高亲和度的Tandem混合Ubiquitin结合域 (ThUBD) 融合蛋白.
涂层 96 孔板密集的 ThUBD 聚变蛋白.
我们将ThUBD平台与Tandem Ubiquitin Binding Entity (TUBE) 涂层板进行了比较.

主要成果:

建立了一个高通量平台,用于识别和量化无处不在的蛋白质.
通过所有类型的无素链来修改的蛋白质的无偏和高亲和度捕获.
与 TUBE 涂层板相比,用于捕获聚基化蛋白质的线性范围扩大了 16 倍.

结论:

覆盖ThUBD的平台提供了有效的检测和精确量化无处不在信号.
支持研究全球无处不在的概况和特定目标的无处不在状态.
提供强大的技术支持,用于开发蛋白质溶解向金马 (PROTACs).

关键词:

高通量检测检测的高通量检测.这就是PROTAC.用 ThUBD 涂层的板块.在Ubiquitination中使用.

更多相关视频

High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis QDB

High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis QDB

Published on: August 21, 2018

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Published on: March 23, 2020

相关概念视频

Western Blotting

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.