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EGCG-蛋黄蛋白复合体-稳定HIPPE用于增强脂友生物活性物质的递送:洞察结构和功能关系

Xinyu Kou¹, Jiahao Bie¹, Jinxuan Cao¹

¹Key Laboratory of Geriatric Nutrition and Health, School of Food and Health, Beijing Technology and Business University, Beijing 100048, China.

|November 6, 2025

概括

蛋白质多复合物增强脂友生物活性递送. 最佳的表甲基酸盐 (EGCG) 度提高了乳液特性和营养物质的生物可访问性,而过多的EGCG则导致不稳定.

科学领域:

食品科学与技术食品科学与技术
体和接口科学科学
营养生物化学营养生物化学

背景情况:

非共价蛋白-多复合物为脂友生物活性递送提供界面稳定和抗氧化益处.
卵黄脂蛋白 (LDL:HDL, 3:7 w/w) 被用作蛋白质来源.
乙甲基酸盐 (EGCG) 是一种具有抗氧化特性的多.

研究的目的:

研究蛋白质-EGCG复合物的形成和特性.
评估这些复合物的应用在稳定高内部相皮克林乳液 (HIPPEs) 中,这些乳液中含有植物 Ester (PE).
为了确定EGCG的最佳度以实现乳液稳定性和生物活性递送.

主要方法:

使用超声处理 (20 kHz, 75 W, 10 分钟) 的卵黄脂蛋白复杂形成,具有不同的EGCG度 (0, 10, 20, 40 μg/mL).
使用光谱和学分析对结构和界面特性进行表征.
在模拟消化过程中评估HIPPE稳定性,滴滴大小,界面粘弹性,泽塔潜力和PE生物可访问性.

主要成果:

EGCG诱导部分蛋白质通过键和疏水相互作用展开,增强界面吸附.
含有10或20μg/mLEGCG的HIPPE显示出最佳特性:滴滴尺寸更小,界面粘弹性改善,高泽塔电位 (31.5mV) 和增加PE生物可访问性.
较高EGCG度 (40微克/毫升) 导致蛋白质聚合,双乳液形成,并影响了乳液稳定性和消化.

结论:

在蛋白质-脂蛋白复合体形成和乳液稳定中,EGCG表现出度依赖的双重作用.
一个最佳的EGCG范围 (10-20μg/mL) 平衡了乳液稳定性,并增强了像植物醇等脂性生物活性物的生物可访问性.
这项研究提供了开发功能乳液的策略,以改善食品应用中的脂性化合物的传递.

关键词:

生物可访问性生物可访问性甲基甲基酸盐 (EGCG) 是一种甲基酸盐.高内部相皮克林乳液 (HIPPE) 是一种高内部相皮克林乳液.脂蛋白复合体是指脂蛋白复合体.非共价相互作用非共价相互作用

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