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Updated: Jan 12, 2026

Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples

Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples

Published on: October 18, 2019

超越盘子:一个广泛的学校的基因组特征Clostridium perfringens类型F爆发

Sylvia Valdezate¹, María J Medina-Pascual¹, César Rodríguez-García²

¹Reference and Research Laboratory for Taxonomy, National Centre of Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases

|November 6, 2025

概括

2023年西班牙一所学校的食物传播疫情 (FBO) 涉及Clostridium perfringens,影响了526人. 基因组分析揭示了两种具有多种毒素的独特菌株,突出了食品安全方面的担忧.

科学领域:

通过食物传播的病原体基因组学
微生物流行病学微生物流行病学
公共卫生微生物学

背景情况:

杆菌是食物传播疫情 (FBO) 的主要原因之一.
了解FBO的基因组和流行病学因素对于公共卫生至关重要.
在2023年,西班牙一所学校发生了显著的FBO.

研究的目的:

调查主要的Clostridium perfringens食品传播疫情的流行病学和基因组特征.
为了确定致病因素和致病菌株的遗传关系.
评估对食品安全的公共卫生影响.

主要方法:

对Clostridium perfringens菌株的大规模胃肠炎爆发的基因组分析.
流行病学调查,包括流行病曲线分析.
鉴定病毒性基因,毒素概况和克隆系 (STs/cgSTs).
体分析和菌体谱的表征.

主要成果:

疫情影响了526人,流行曲线为单模式,可能源自受污染的博洛尼亚汁.
确定了两个不同的克隆系 (ST-804/cgST-804和ST5/cgST-801).
这些菌株具有广泛的毒性,包括CPA,CPE,CPB2和perfringolysin等毒素.
肠毒素基因 (cpe) 发现在可调动的等离子体上,与F型菌株的典型染色体位置不同.
对甲尼达和万科米辛的抗生素敏感性保持不变.
这两种血统都表现出独特的菌体谱.

结论:

这项研究代表了西班牙第一个Clostridium perfringens FBO的基因组调查.
研究结果表明,在同一爆发中,可能存在多个污染源.
结果强调了与大型公共设施的食品安全相关的重大公共卫生风险.

关键词:

贝塔2毒素是一种毒素.克洛斯特里透,F型食物传播疾病食物传播疾病疫情爆发的原因是青醇氨基酸 o 青醇氨基酸塑体内毒素 (enterotoxin) 是一种有毒的物质.学校学校学校学校在校

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