利用向测序来对不同样本的肺部感染进行病因诊断
Xiaojun Guan1, Kaisar Gufur1, Liangliang Xu1
1Department of Thoracic Surgery, Xinjiang Uygur Autonomous Region Sixth People's Hospital, Urumqi, Xinjiang Uygur Autonomous Region, China.
Frontiers in cellular and infection microbiology
|November 7, 2025
概括
针对性下一代测序 (tNGS) 为肺部感染提供了卓越的诊断方法,比传统方法检测出更多的病原体. 这种先进的技术可以改善病原体的识别,并有助于管理复杂的病例.
科学领域:
- 医学诊断 医学诊断 医学诊断
- 传染性疾病 传染性疾病
- 基因组学就是基因组学.
背景情况:
- 肺部感染是一个重大的临床挑战,往往需要快速准确的病原体识别才能有效治疗.
- 传统的病原体检测方法 (TPD) 在灵敏度,检测频谱和无法识别病毒病原体或混合感染方面存在局限性.
研究的目的:
- 评估针对性下一代测序 (tNGS) 的诊断价值,用于识别肺部感染患者的各种样本类型中的病原体.
- 将tNGS与传统病原体检测方法 (TPD) 的性能进行比较.
主要方法:
- 一组由166名疑似肺部感染的患者组成的队列提供了包括支气管洗液,组织,多发性溢液和唾液在内的样本.
- 使用tNGS和TPD进行了病原体检测.
- 对tNGS和TPD之间的诊断性能进行了比较分析.
主要成果:
- 与TPD相比,tNGS的阳性检测率 (81.33%) 显著更高 (32.53%).
- 与TPD相比,tNGS发现了更广泛的病原体,包括35种细菌,10种真菌和18种病毒物种,而TPD只发现了11种细菌和2种真菌物种.
- tNGS有效地检测到混合感染,并确定了16个抗生素耐药性基因 (ARG),这是TPD所无法提供的功能.
结论:
- tNGS为肺部感染提供了更高的灵敏度和更广泛的病原体检测谱.
- tNGS能够识别混合感染和抗生素耐药性基因的能力使其成为临床实践中一个有价值和可靠的替代诊断工具.
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