取决于ubiquitylation的Rap2激活可以调节细胞迁移期间的lamellipodia动态
Andrew Neumann1, Revathi Sampath1,2, Emily Mayerhofer1
1Department of Cell and Developmental Biology, School of Medicine , University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Journal of cell science
|November 7, 2025
概括
拉普2蛋白被招募到称为lamellipodia的细胞结构中,在那里它调节了actin动态,以帮助细胞迁移. 这个过程需要Rap2激活和向细胞膜的无处不在.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 细胞迁移依赖于actin细胞骨架动力学和细胞极性.
- 拉斯GTPase的Rap2通过Rab40/CRL5.5的无处不在来定位到拉梅利波底.
- 在细胞迁移中,Rap2的确切功能和向机制尚不清楚.
研究的目的:
- 阐明Rap2在细胞迁移中的作用.
- 为了了解Rap2是如何向拉美利波底的.
- 调查在Rap2激活和定位中无处不在的作用.
主要方法:
- 使用了遗传学和药理学方法.
- 调查了Rap2招募Lamellipodia的情况.
- 分析了Rap2与RhoA和ARHGAP的相互作用29.
- 检查了Rab40/CRL5依赖性无处不在化对Rap2激活和膜向的要求.
主要成果:
- 拉普2抑制RhoA,可能是通过ARHGAP29来抑制拉梅利波迪亚的卷纹.
- 拉普2调节了lamellipodia动态,从而促进了细胞迁移.
- Rab40/CRL5依赖的无处不在化对于依赖于关氨酸核酸交换因子 (GEF) 的Rap2激活至关重要.
- 在Rap2向乳皮质膜时,需要泛基化.
结论:
- 在细胞迁移过程中,Rap2在调节lamellipodia actin动态方面发挥着至关重要的作用.
- 通过Rab40/CRL5介导的无处不在,独特地激活和向Rap2到拉美利波底.
- 这项研究揭示了影响细胞运动的Rap2调节的新机制.
相关概念视频
Mechanism of Lamellipodia Formation
3.5K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
3.5K
Cell Polarization by Rho Proteins
3.5K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
3.5K
Cytoskeletal Coordination in Cell Migration
5.4K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
5.4K
Intracellular Signaling Affects Focal Adhesions
3.5K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
3.5K
Mechanism of Filopodia Formation
3.0K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
3.0K
Cell Migration
18.5K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
18.5K


