相关实验视频

Updated: Jul 20, 2026

Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System
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Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System

Published on: May 28, 2015

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使用CRISPR激活快速验证人类多能干细胞中静态基因记者的协议

Youjun Wu1, Aaron Zhong1, Bernny Ramirez1

  • 1The SKI Stem Cell Research Facility, The Center for Stem Cell Biology and Developmental Biology Program, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065, USA.

STAR protocols
|November 7, 2025
PubMed
概括

这项研究引入了快速的CRISPR介导转录激活 (CRISPRa) 工作流程,以验证人类多能干细胞 (hPSCs) 中的记者基因敲定. 这种方法有效地验证了在静态基因位点的记者表达,简化了干细胞研究.

关键词:
克里斯普尔是什么意思?克里斯普尔是什么意思?干细胞是干细胞.组织工程是组织工程.

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