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基于单分子实时测序,构建了来自水下尸体的水中的微生物组的继承模型
Xiaoxin Hu1, Mengqi Wu2, Bolei Zhao1
1School of Investigation, People's Public Security University of China, Beijing 100038, China.
Forensic science international. Genetics
|November 7, 2025
概括
这项研究跟踪了水中浸泡的老鼠尸体周围的微生物变化,以估计死后浸泡间隔 (PMSI). 确定了关键微生物,导致MAE低于6天的模型,以准确估计分解时间.
科学领域:
- 法医科学 法医科学 法医科学
- 微生物学 微生物学
- 环境科学 环境科学
背景情况:
- 在水生环境中估计死后浸水间隔 (PMSI) 是具有挑战性的,因为传统方法的局限性.
- 了解微生物继承对于制定准确的水生分解时间框架至关重要.
研究的目的:
- 为了研究水中微生物的继承模式,周围的水下尸体.
- 评估这些微生物模式对估计PMSI的潜力.
- 开发基于水中的微生物组的PMSI预测模型.
主要方法:
- 在河流和自来水中浸泡的老鼠尸体70天.
- 在多个时间点 (0-70天) 收集水样.
- 使用单分子实时测序分析微生物社区动态.
主要成果:
- 鉴定了在分解阶段普遍存在的关键微生物,尽管水源之间存在差异.
- 在物种层面上表征微生物社区的继承.
- 开发了PMSI估计的随机森林模型,平均绝对误差为5.87 (河流) 和4.86 (水龙头) 天.
结论:
- 水中的微生物继承为水生PMSI估计提供了可行的指标.
- 这项研究为在法医科学中使用水微生物组建立了框架.
- 这些发现支持了多功能PMSI推断模型的进一步开发.
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