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通过CRISPR/Cas9系统生成Cre/LoxP介导的细胞外TurboID敲进大鼠

Sara Ito1, Katsuyuki Nakamura2, Kazuya Murata3

  • 1Department of Life Science and Chemistry, Graduate School of Natural Science and Technology, Gifu University, Gifu, Japan.

Biochemical and biophysical research communications
|November 7, 2025
PubMed
概括

研究人员开发了用于细胞表面生物化的TurboID敲门老鼠. 这种新模型能够在体内分析细胞外蛋白质,有助于发现药物标和抗原.

关键词:
生物化是生物化的一种.在Cre/LoxP系统中.敲进来的老鼠.这是一个TurboIDID.

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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