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循环RNA Gsr-0002通过调节DNMT3A/PTEN通路来促进分裂体诱导的肝纤维化

Bingxin Dai¹, Sijia Huang², Qingyue Hu³

¹Department of Microbiology and Parasitology, Anhui Province Key Laboratory of Zoonoses, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui, 230032, People's Republic of China; Department of Clinical Laboratory, South China Hospital, Medical School, Shenzhen University, Shenzhen, Guangdong, 518116, People's Republic of China.

International journal of biological macromolecules

|November 7, 2025

概括

循环RNAs (circRNAs) 起到作用,在schistosomiasis诱导的肝纤维化. circGsr-0002通过隔离miR-383-3p促进肝星细胞激活和纤维化,提供潜在的治疗点.

科学领域:

分子生物学分子生物学
细胞生物学细胞生物学
寄生虫学的寄生虫学

背景情况:

肝纤维化是istosomiasis的一种严重并发症.
循环RNAs (circRNAs) 涉及各种疾病,但它们在分裂体诱导的肝纤维化中的作用尚不清楚.

研究的目的:

为了研究肝星细胞 (HSCs) 中的circRNAs的表达特征和功能,在阴茎症诱导的肝纤维化期间.
确定特定的circRNA及其涉及HSC激活和肝纤维化进展的分子机制.

主要方法:

来自 Schistosoma japonicum 感染小鼠的初级 HSC 中circRNAs 的高通量测序.
定量实时PCR (qRT-PCR) 用于验证差异表达的circRNAs.
在体外测试包括RNA免疫沉降,双露西法酶记者测试和细胞转染,以阐明分子相互作用和功能.

主要成果:

489个显著差异表达的circRNA被确定在感染小鼠的HSC中.
circGsr-0002在受感染的HSC和肝脏组织中被上调,促进HSC的激活,增殖和抑制亡.
circGsr-0002与miR-383-3p相互作用,调节PTEN通路并导致肝纤维化.

结论:

circGsr-0002是一种关键的circRNA,用于发生杆菌病引起的肝纤维化.
该circGsr-0002/miR-383-3p/PTEN轴是一个新的途径,驱动HSC激活和纤维化.
循环RNAs代表了治疗肝纤维化的潜在治疗点.

关键词:

电路Gsr-0002 的情况.循环RNAs是一种循环RNA.在DNMT3A中,它是DNMT3A.肝脏纤维化肝脏纤维化在这里,我们可以使用PTEN.在 miR-383-3p 中.

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