在Streptomyces coelicoloror中通过accA1/accA2功能分歧介导的组装动态和代谢流量再分配
Shiyu Wu1, Ximing Chen2, Yujie Wu2
1State Key Laboratory of Cryospheric Science and Frozen Soil Engineering, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, 730000, China; Key Laboratory of Extreme Environmental Microbial Resources and Engineering of Gansu Province, Lanzhou, 730000, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
International journal of biological macromolecules
|November 7, 2025
概括
这项研究表明,Streptomyces coelicolor中的两个α子单元基因,accA1和accA2,在新陈代谢中发挥着不同的作用. AccA1引导碳流向二次代谢物,而AccA2支持初级代谢和脂肪酸合成.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 代谢工程是代谢工程.
背景情况:
- 乙-CoA核糖酶 (ACC) 复合体对于Streptomyces中的脂肪酸和多基化物生物合成至关重要.
- 斑点菌 (Streptomyces coelicolor) 具有两个同源的α子单元基因,accA1和accA2,参与ACC功能,但它们的不同作用尚不清楚.
研究的目的:
- 在Streptomyces coelicolor M145.5中系统地描述accA1和accA2之间的功能分歧.
- 阐明这些基因如何差异调节碳流向初级和二级新陈代谢.
主要方法:
- 使用qPCR和西式涂抹进行基因表达概况.
- 生理分析和代谢分析.
- 同免疫沉 (Co-IP) 分析用于研究蛋白质复合体组合.
主要成果:
- 观察到accA1和accA2的不同表达模式.
- 过度表达accA2增强了生物质和初级代谢,而accA1过度表达扰乱了初级代谢并诱导了二次代谢物 (actinorhodin) 的产生.
- 代谢数据证实了accA2在初级代谢中的作用和accA1在二次代谢中的作用.
- 联合IP测试显示,AccA2复合体是稳定的,而AccA1复合体更灵活,并优先形成ACC.
结论:
- 通过不同的表达和组装动态,AccA1和AccA2表现出功能分歧,通过不同的表达和组装动态不同调节碳流.
- 主要通过AccA1引导碳流向二次代谢物生物合成.
- AccA2主要支持脂肪酸和其他初级代谢过程,为Streptomyces的代谢工程提供了基础.
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