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通过大麻素受体1传递的Delta 9-Tetrahydrocannabinol信号改变了热囊细胞的分化

Jessica L Koven¹, Bryony V Natale¹, Daniel B Hardy^2,3,4,5

¹Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada.

Stem cells and development

|November 7, 2025

概括

怀孕期间接触大麻可能会损害胎盘发育. 德尔塔-9-THC通过大麻素受体1 (CB1) 影响热囊细胞分化,可能影响胎儿生长.

科学领域:

生殖生物学生殖生物学
发展性毒理学发展性毒理学
药理学药理学是指药理学的学科.

背景情况:

怀孕期间大麻的使用量正在上升,这引发了对胎儿暴露于delta-9四大麻 (Δ9-THC) 的担忧.
动物研究将产前D9-THC暴露与胎盘病理联系起来,特别是迷宫发育受损.
大麻素受体1 (CB1) 调解 Δ9-THC 效应,但其在胎盘中的精确表达是有争议的.

研究的目的:

在动物的胎盘中映射CB1/Cnr1的时空表达.
为了确定CB1是否调解了多细胞分化中的Δ9-THC诱导的变化.
调查 Δ9-THC 对胎盘生长的影响机制.

主要方法:

免疫组织化学检测胎盘组织中的CB1表达.
在实验室中使用小鼠热囊细胞干细胞 (mTS) 的研究,包括CB1淘汰 (mTS^Cnr1^KO) 模型.
在暴露于Δ9-THC后,对热细胞分化标志物 (增殖,分化和特种细胞类型) 的分析.

主要成果:

与之前的报道相反,CB1在所有面向血液的母体热囊细胞中得到表达.
Δ9-THC暴露显著改变了分化热细胞的标记物,而不是增殖细胞.
以CB1依赖的方式表达 Δ9-THC 损害的同聚氨基体 (SynT) 标记物,促进结区/体巨细胞分化.

结论:

CB1 / CNR1表达在胎盘中比以前认为的更为普遍.
Δ9-THC破坏了对胎盘发育至关重要的热囊细胞分化途径.
这项研究揭示了一种潜在的机制,即产前大麻暴露可能对胎盘生长和胎儿发育产生不利影响.

关键词:

CB1 CB1 CB1 的意思是什么意思.在Cnr1中.传输系统细胞 TS细胞 TS细胞大麻素受体 1 1 大麻素受体大麻大麻大麻大麻大麻大麻大麻大麻胎盘胎盘胎盘胎盘综合性类细胞.热囊细胞的热囊细胞.热囊细胞分化的差异化热囊细胞干细胞的干细胞在 Δ9-THCC 中.

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