Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Keto–Enol Tautomerism: Mechanism01:14

Keto–Enol Tautomerism: Mechanism

7.6K
The keto and enol forms are known as tautomers and they constantly interconvert (or tautomerize) between the two forms under acid or base catalyzed conditions. Both the reactions involve the same steps—protonation and deprotonation— although in the reverse order.
7.6K
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

11.0K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
11.0K
Other Glycolytic Pathways01:24

Other Glycolytic Pathways

794
The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
794

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The discovery of new metagenomic urethanases utilising a novel colorimetric assay for applications in the biodegradation of polyurethanes.

Green chemistry : an international journal and green chemistry resource : GC·2025
Same author

Expanding the Enzymatic Toolbox for Carboligation: Increasing the Diversity of the 'Split' Transketolase Sequence Space.

Chembiochem : a European journal of chemical biology·2025
Same author

Stereodivergent Biocatalytic Formal Reduction of α-Angelica Lactone to (R)- and (S)-γ-Valerolactone in a One-Pot Cascade.

Chembiochem : a European journal of chemical biology·2023
Same author

Assessing and Engineering Antibody Stability Using Experimental and Computational Methods.

Methods in molecular biology (Clifton, N.J.)·2022
Same author

Synthesis and Characterization of Optically Active Surfactants Derived from Phenylalanine and Leucine.

Journal of colloid and interface science·2001

相关实验视频

Updated: Jan 12, 2026

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
10:10

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Published on: September 20, 2016

14.9K

转基因酶酶的发现和工程.

Alessia Tonoli1, Paul Anthony Dalby1, Helen Claire Hailes2

  • 1Department of Biochemical Engineering, University College London, London, United Kingdom.

Methods in enzymology
|November 7, 2025
PubMed
概括

转基因酶 (TKs) 是对C-C键形成至关重要的酶. 正在发现并为生物催化剂设计具有改变基质特异性和增强稳定性的新型TK变体.

关键词:
生物信息学是一种生物信息学.发现了酶的发现.酶进化 酶进化的过程转基因组学是指转基因组学.转基因托拉酶的转基因托拉酶是什么

更多相关视频

Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis
13:31

Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis

Published on: October 31, 2014

14.4K
A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications
07:59

A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications

Published on: September 10, 2021

4.6K

相关实验视频

Last Updated: Jan 12, 2026

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
10:10

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Published on: September 20, 2016

14.9K
Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis
13:31

Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis

Published on: October 31, 2014

14.4K
A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications
07:59

A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications

Published on: September 10, 2021

4.6K

科学领域:

  • 生物化学 生物化学
  • 酶学 是一种酶学.
  • 代谢工程是代谢工程.

背景情况:

  • 转基因酶 (TKs) 是利用特定的化糖形成C-C键的关键酶.
  • 生物催化应用通常使用β-基酸作为捐赠基质.
  • 对于具有独特性质和扩展基质范围的新型技术技术的需求日益增长.

研究的目的:

  • 为了提供TCK基板的概述.
  • 详细介绍用于发现新型TC的元基因组策略,包括"分裂"TC.
  • 描述酶进化方法,以改进稳定性和基质接受度的工程TK.

主要方法:

  • 为新型转基因酶发现进行元基因组查.
  • 酶突变发生和定向进化技术.
  • 基质接受和酶稳定性的表征.

主要成果:

  • 通过元基因组方法识别独特的跨基酶.
  • 通过突变发生的改变了基质配置的TC的工程.
  • 开发具有增强生物催化剂稳定性的TCK变体.

结论:

  • 甲基因组学和酶进化是发现和工程转基因酶的强大工具.
  • 工程化TCK为生物催化应用提供了扩展的基质范围和更好的稳定性.
  • 进一步的研究将继续扩大TK在合成生物学和生物技术中的实用性.