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Updated: Jan 12, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

在肠道衰竭相关的肝病中,全基因组识别异常的替代拼接和RBP调节器

Tao Zhang¹, Xinyi Wang², Sai Yang³

¹Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Key Laboratory of Anesthesiology and Resuscitation (Huazhong University of Science and Technology), Ministry of Education, China; Institute of Anesthesia and Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.

Arab journal of gastroenterology : the official publication of the Pan-Arab Association of Gastroenterology

|November 7, 2025

概括

替代拼接变化是肠道衰竭相关的肝病 (IFALD) 的关键,来自全方位营养 (TPN). 这种Mbnl3-Zmynd11通路显示出IFALD治疗的潜力.

科学领域:

肝病学肝病学是一种肝病学.
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

肠道衰竭相关的肝病 (IFALD) 是全肠直肠营养 (TPN) 的严重并发症.
驱动IFALD病变的精确分子机制在很大程度上是未知的.
了解这些机制对于改善TPN依赖患者的治疗结果至关重要.

研究的目的:

调查替代拼接 (AS) 在IFALD发展中的作用.
为了确定特定的AS事件和参与IFALD病变的监管网络.
探索IFALD的潜在治疗点.

主要方法:

分析了从老鼠肝脏样本 (TPN诱导的IFALD与对照) 的转录组数据.
使用SUVA管道量化了替代拼接和调节的替代拼接 (RAS) 事件.
评估了差异性基因表达,巨细胞透和RNA结合蛋白 (RBP) 网络.

主要成果:

确定了268个主要的IFALD-RAS事件,主要与细胞粘附途径有关.
在IFALD肝脏中观察到M2巨细胞透的增加,与特定的AS事件相关.
建立了一个涉及33个差异表达的RBP和RAS事件的监管网络.

结论:

替代拼接变化与IFALD.的病原发生有关.
Mbnl3-Zmynd11轴代表了管理IFALD的潜在治疗目标.
对AS调节的进一步研究可能为TPN诱导的肝脏并发症提供新的治疗策略.

关键词:

另一个替代拼接.免疫系统的免疫系统.与肠道衰竭相关的肝病.父母肠道营养是一种营养.这是一种RNA结合蛋白.

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相关概念视频

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...