野生蜂Ceratina calcarata候选参考基因的发育和环境稳定性
Zixiao Zhao1, Rameshwor Pudasaini1, Camiyah Woods1,2
1Agricultural Research Development Program, Central State University, Wilberforce, OH, USA.
Scientific reports
|November 7, 2025
概括
研究人员确定了RPS18和RPL8作为小木匠蜜蜂Ceratina calcarata中定量实时PCR (q-RT-PCR) 最稳定的参考基因. 这确保了用于授粉者研究的精确基因表达分析.
科学领域:
- 生态生态学 生态生态学
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 准确的基因表达分析对于理解生物过程至关重要.
- 定量实时PCR (q-RT-PCR) 依赖于稳定的参考基因进行正常化.
- 小木匠蜜蜂 (Ceratina calcarata) 是一个生态上重要的野生蜜蜂物种.
研究的目的:
- 为了确定和验证稳定的基因参考基因基因表达研究在Ceratina calcarata.
- 确保可靠的数据规范化,跨越不同的发展阶段和环境条件.
- 为了支持未来的授粉者生物学和保护基因组学研究.
主要方法:
- 评估了七个候选参考基因 (RPS18,RPS5,RPL32,RPL8,EF-1α,β-Actin,GAPDH).这些基因的基因组分别是:
- 分析了基因表达在不同发育阶段 (,,成年人) 和景观环境 (农场,路边站点) 的稳定性.
- 使用了四个分析算法 (GeNorm,NormFinder,BestKeeper,ΔCt方法) 和RefFinder进行全面验证.
主要成果:
- RPS18和RPL8被确定为Ceratina calcarata最稳定的参考基因.
- 基因表达稳定性在各种生物和环境变量中进行了评估.
- 这些发现得到了多种分析方法的一致支持.
结论:
- 在Ceratina calcarata中,RPS18和RPL8是准确的q-RT-PCR的验证参考基因.
- 这项研究提供了必要的工具,可靠的基因表达分析在这个野生蜜蜂物种.
- 经过验证的参考基因将促进未来关于授粉者健康,环境反应和保护的研究.
更多相关视频
相关概念视频
02:02Cis-regulatory Sequences
11.6K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.6K
02:27Background and Environment Affect Phenotype
7.4K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
7.4K