基于MnO2纳米酶和碳点的多式传感平台,用于色度和光检测过氧酸盐
Man Du1, Shuang Hou1, Xinyue Yang1
1College of Chemical and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang, 050018, P. R. China.
Mikrochimica acta
|November 7, 2025
概括
这项研究介绍了一种新的多模式传感平台,用于使用二氧化纳米板和碳点检测过氧化 (ONOO-). 开发的方法可以在生物样本和活细胞中灵敏,经济和方便地检测ONOO-.
科学领域:
- 生物医学工程 生物医学工程
- 分析化学 分析化学
- 材料科学 材料科学 材料科学
背景情况:
- 过氧化 (ONOO-) 是一种关键分子,与各种疾病有关.
- 准确可靠地检测ONOO-对于疾病诊断和研究至关重要.
- 现有的检测方法在灵敏度,特异性或实用性方面可能存在局限性.
研究的目的:
- 开发一种新的色度和度多模式传感平台,用于定量ONOO检测.
- 使用二氧化纳米片 (MnO2 NSs) 和碳点 (CDs) 来增强传感能力.
- 为了实现对生物样本和活细胞中的ONOO-的敏感,经济和方便的检测.
主要方法:
- 开发一个集成MnO2 NS和CD的传感平台.
- 使用TMB被MnO2NS氧化,然后被ONOO-氧化进行色度检测.
- 在CD和氧化TMB之间使用光共振能量转移 (FRET) 或直接与ONOO-进行光检测.
- 与智能手机技术集成,用于视觉,现场检测.
- 在人类血清样本和活细胞光成像中的应用.
主要成果:
- 多模式传感平台在ONOO检测方面表现出极好的灵敏度.
- 测色模式 LOD: 28.73 nM (1-70 μM范围). 测色方式 LOD: 28.73 nM (1-70 μM范围).
- 度模式的LODs: 40.17nM (0.3-45μM范围) 和25.47nM (0.3-55μM范围).
- 通过智能手机成功进行视觉现场检测.
- 在人血清样本中,复原率很高 (95.5%-104.7%).
- 在活细胞中有效光成像ONOO-.
结论:
- 开发的多式传感平台在单式检测方法上提供了显著的进步.
- 该平台为ONOO-检测提供了一种经济,高效和方便的方法.
- 这种方法对临床诊断,疾病研究和生物成像应用具有前景.
- MnO2 NS和CD的整合创造了一个具有广泛适用性的多功能传感系统.
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