修改后的S-K方法和ICP-MS用于尿测量的比较:一致性,准确性和维生素C干扰
Xiufang Shi1, Xiaoqing Zhang, Xuerong Liu
1Laboratory Center, Bozhou People's Hospital, Bozhou City, Anhui Province, China.
Medicine
|November 8, 2025
概括
经过修改的桑德尔-科尔托夫测定方法显示,与ICP-MS测量尿的总体一致性很好. 然而,高维生素C水平会干扰S-K测定,导致不准确的结果.
科学领域:
- 生物化学 生化学
- 分析化学 分析化学
- 公共卫生 公共卫生
背景情况:
- 准确的状态测量对于公共健康至关重要,特别是对于孕妇和患有甲状腺疾病的人来说.
- 尿液中的度是评估状态的关键指标.
- 测量尿度的现有方法需要对准确性和潜在干扰进行验证.
研究的目的:
- 为了确定修改后的桑德尔-科尔托夫 (S-K) 试验和诱导合血质谱法 (ICP-MS) 之间的一致性,用于测量尿液度.
- 评估不同维生素C (Vc) 水平对修改后的S-K测定与ICP-MS.相比对其准确性的影响.
- 在存在潜在干扰物质的情况下,评估修改后的S-K方法的可靠性.
主要方法:
- 用修改的S-K和ICP-MS方法对155个尿样进行前性分析,并进行三次测量.
- 基于定性维生素C水平的样本分类:低 (-或±),中等 (+至2+) 和高 (3+).
- 相关性和布兰德-阿尔特曼分析来比较这两种方法并评估维生素C干扰.
主要成果:
- 修改后的S-K方法与ICP-MS的整体一致性很好 (R=0.458,P<0.01).
- 与ICP-MS相比,修改后的S-K方法产生了38.189μg/L的更高的尿平均度 (P<0.05).
- 随着维生素C水平的增加,相关性下降 (R=0.677,0.655,0.494分别为低,中,高Vc组),高Vc导致S-K方法中的积极偏差.
结论:
- 虽然修改后的S-K方法与ICP-MS显示出良好的总体一致性,但它容易受到高维生素C水平的干扰.
- 修改后的S-K试验可能会高估尿的度,特别是在摄入维生素C高的人群中.
- 需要进一步完善S-K方法,以减轻维生素C干扰,以准确评估状态.
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