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索尔比托的吸收和氧的转移形状AOX1促进剂诱导在格式脱酶-缺陷的Komagataella phaffii

Cristina Bustos^1,2, Rocio Cozmar^1,2, Julio Berrios²

¹Microbial Processes and Interactions, TERRA Teaching and Research Centre, Gembloux Agro-Bio Tech, University of Liege, Gembloux, Belgium.

Microbial biotechnology

|November 8, 2025

概括

来自单碳代谢的形式可以诱导Komagataella phaffii中的P_AOX1表达. 增强索比代谢减少了这种诱导,突出了碳源利用和重组蛋白质生产之间的复杂相互作用.

科学领域:

生物技术是生物技术.
分子生物学分子生物学
生物化学生物化学

背景情况:

格式作为一个替代诱导器的AOX1促进器 (P_AOX1) 在Komagataella phaffii,提供一个没有甲醇的表达系统.
酸盐积聚在酸盐脱酶缺乏 (FdhKO) 的K. phaffii菌株中,在酸盐上生长,使得与甲醇诱导系统相比的重组蛋白质生产成为可能.
在K. phaffii中,索尔比托尔代谢是低效的,可能会限制生长和重组蛋白质产量.

研究的目的:

通过在FdhKO菌株中过度表达酸脱酶 (SOR1) 来增强K. phaffii中的酸醇代谢.
调查改善的醇代谢对P_AOX1诱导和重组蛋白质生产力的影响.
探索氧气供应对这些代谢和表达动态的影响.

主要方法:

在缺乏格式脱酶 (FdhKO) 的 Komagataella phaffii 菌株中,SOR1 基因过度表达.
在不同氧气转移条件下,在无甲醇,基媒介中培育工程菌株.
量化形式积累,生物质形成和重组蛋白质 (eGFP,CalB脂酶,Gox) 生产.

主要成果:

过度表达SOR1改善了醇代谢和生物质形成,但降低了P_AOX1诱导,导致较低的复合蛋白产量.
减少的氧气转移条件促进了FdhKO菌株中更高的P_AOX1诱导和形式积累.
低氧转移和低酸盐吸收率的组合增加了诱导细胞的比例.

结论:

增强的醇代谢对K. phaffii.中P_AOX1介导的基因表达产生负面影响.
氧气的可用性是调节碳代谢和基因诱导之间的相互作用的关键因素.
优化酸醇吸收和氧气转移对于改善K. phaffii. 中的重组蛋白质生产策略至关重要.

关键词:

科玛格塔贝拉 (Komagataella phaffii) 是一个有趣的植物.一个AOX1促进者.格式格式格式格式形成脱基酶的形式氧气转移速率是指氧气的转移速率.索尔比托的吸收方式

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