来自Amphioxus的依赖的细菌结合性C型乳素的功能性特征.
Huiqing Huang1, Enjin Deng2, Dandan Xu1
1Guangdong Food and Drug Vocational College, No. 321 Longdong North Road, Tianhe District, Guangzhou 510520, China.
International immunopharmacology
|November 8, 2025
概括
我们鉴定了AmphiCTL6,这是一种新型的C型讲蛋白 (CTL) 在amphioxus中,作为一个广谱模式识别受体 (PRR). 它结合了各种细菌,突出了其在天生的免疫力和肠道监测中的作用.
科学领域:
- 这是天生的免疫力.
- 比较免疫学比较免疫学
- 海洋生物学 海洋生物学
背景情况:
- C型讲解蛋白 (CTL) 是先天免疫中的关键模式识别受体 (PRR).
- 安菲奥克萨斯拥有大量的CTL基因,但很少有功能性特征.
- 了解像安菲奥克斯 (Amphioxus) 这样的基底带动物的CTL功能,可以了解免疫进化.
研究的目的:
- 描述一种来自Branchiostoma japonicum (B. japonicum) 的新型CTL,即AmphiCTL6.
- 研究AmphiCTL6.6的免疫功能和结合能力.
- 探索AmphiCTL6在amphioxus的先天免疫系统中的作用.
主要方法:
- 基因预测和基因组分析确定了AmphiCTL6.6.
- 在现场杂交和单细胞RNA-seq确定了AmphiCTL6的表达模式.
- 进行了重组蛋白表达,碳水化合物结合测定,细菌聚合和域切断分析.
主要成果:
- AmphiCTL6具有独特的结构,具有协同的EGF类域和CTLD.
- 在肝,肠道,和性腺中观察到高表达,特别是在肠道上皮细胞中.
- AmphiCTL6证明了各种细菌的依赖的结合和凝结,CTLD调解了这些相互作用.
结论:
- AmphiCTL6 作为 amphioxus中的广谱模式识别受体 (PRR) 起作用.
- 这一发现扩大了已知的基底带动物中CTL的功能多样性.
- AmphiCTL6在肠道免疫监测和天生的免疫力中发挥着重要作用.
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