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Updated: Jan 12, 2026

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

解码宿主免疫反应:Klebsiella variicola感染的子脏中的circRNAs

Lei Yin¹, Xuehuai Shen¹, Dongdong Yin¹

¹Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Sciences, Hefei, Anhui 230031, China; Anhui Province Key Laboratory of Livestock and Poultry Product Safety, Hefei, Anhui 230031, China.

Veterinary microbiology

|November 8, 2025

概括

这项研究揭示了循环RNA (circRNAs) 是对Klebsiella variicola感染的免疫反应的关键参与者. 这些circRNAs调节先天性免疫和炎症,为抗感染策略提供新的点,以对抗这种食源性病原体.

科学领域:

* 分子生物学 * 分子生物学
* 免疫学免疫学
* * 食品安全食品安全

背景情况:

*Klebsiella variicola (K. variicola) 是一种新兴的食物传播病原体,具有动物感染潜力.
*循环RNAs (circRNAs) 参与宿主-病原体相互作用,但它们在K. variicola感染中的作用尚不清楚.
*了解宿主circRNA反应对于开发有效的抗感染策略至关重要.

研究的目的:

* 在K. variicola感染期间,研究宿主circRNAs在的脏中的作用.
* 识别差异表达的circRNA及其相关的生物通路.
*阐明与先天免疫有关的circRNA介导的调节网络.

主要方法:

*使用高通量测序来分析K. variicola感染后的子脏中的circRNA转录档案.
*基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径分析在差异表达的circRNAs上进行.
*使用现有的mRNA和microRNA测序数据构建了竞争的内源RNA (ceRNA) 网络.

主要成果:

* 总共有53个circRNAs在对K. variicola感染的反应中发生了显著的改变 (22个上调,31个下调).
*DE circRNAs主要参与先天性免疫和炎症反应.
*确定了两个ceRNA轴,circRNA_4562/miR-3537/CARD11和circRNA_0366/miR-129-5p/ITPR2,突出显示circRNAs是分子海绵.

结论:

*CircRNAs在鸟类对K. variicola感染的天生的免疫反应中发挥着重要作用.
*已识别的ceRNA网络为了解和对抗K. variicola提供了新的分子标.
*这些发现推进了针对细菌感染的基于ncRNA的抗感染策略.

关键词:

循环RNA 是一个循环RNA.竞争的内源性RNA分析.感染感染感染克莱布西拉 (Klebsiella variicola) 是一种植物.脏脏是指脏的部分.

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