在CSF和血清中检测和分子表征感染性脑炎病毒,与疾病严重程度相关
Magnus Klinteskog1, Anita Koskela von Sydow2, Naveed Asghar3
1Centre for Clinical Research and Education, Region Värmland, Karlstad, Sweden; Department of Clinical Microbiology, Uppsala University Hospital, Uppsala, Sweden.
概括
在患者样本中检测到感染性脑炎病毒 (TBEV) RNA,并与严重疾病有关. 基因测序揭示了来自不同地区的TBEV菌株的变异,影响了公共卫生监测.
科学领域:
- 病毒学 病毒学
- 传染性疾病 传染性疾病
- 分子生物学分子生物学
背景情况:
- 传染性脑炎 (TBE) 是一种由TBE病毒 (TBEV) 引起的重大神经疾病.
- 早期检测和TBEV的遗传特征对于了解疾病传播和严重程度至关重要.
研究的目的:
- 在TBE患者的临床样本中检测TBEVRNA.
- 通过使用桑格序列测序,对检测到的TBEV RNA进行基因特征.
- 调查TBEV RNA检测与疾病严重程度之间的关联.
主要方法:
- 从137名TBE患者 (2016-2021) 的血清和脑脊液 (CSF) 样本进行分析.
- 在血清和脑脊液中使用PCR检测TBEVRNA.
- 在TBEV RNA的5'非编码区域 (5'NCR) 的桑格测序.
- 根据ICU入院,持续时间和机械通风来评估疾病严重程度.
主要成果:
- 在7.3%的患者中检测到TBEVRNA (血清和CSF).
- 可检测的RNA与年龄较大,ICU入院增加和机械通风相关.
- 测序揭示了与参考菌株相比,TBEV 5'NCR的遗传变异,Örebro和Värmland序列之间存在差异.
结论:
- 在临床样本中检测TBEV RNA是可行的,并表明更严重的疾病.
- 对TBEV 5'NCR的遗传分析突出了区域差异,这对流行病学研究很重要.
- 这些发现有助于改进TBE诊断和监测策略.
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