Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

5.4K
Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
5.4K
Membrane Fluidity01:23

Membrane Fluidity

172.3K
Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.
172.3K
Membrane Fluidity01:26

Membrane Fluidity

14.4K
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
14.4K
Multi-pass Transmembrane Proteins and β-barrels01:09

Multi-pass Transmembrane Proteins and β-barrels

6.4K
In multi-pass transmembrane proteins, the polypeptide chain crosses the membrane more than once. The transmembrane polypeptide chain either forms an α-helix or β-strand structure. α-Helix containing multi-pass transmembrane proteins are ubiquitous, whereas β-strand containing ones are mainly found in gram-negative bacteria, mitochondria, and chloroplasts.
α-Helix containing multi-pass transmembrane proteins
Multi-pass transmembrane proteins such as...
6.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Inhibition of ADSS2-mediated de novo AMP biosynthesis re-sensitizes acute myeloid leukemia to BH3 mimetics.

Nature cancer·2026
Same author

CholBindNet as an interpretable neural network for cholesterol-binding site classification.

Communications chemistry·2026
Same author

Evolutionary diversity and structural dynamics of the outer membrane protein Ail in <i>Yersinia</i>.

Journal of biomolecular structure & dynamics·2026
Same author

Prediction of bacterial protein-compound interactions with only positive samples.

Bioinformatics (Oxford, England)·2026
Same author

A two-step clockwork mechanism opens a proteo-lipidic pore in PIEZO2.

Nature chemical biology·2026
Same author

Dynamic nature of Staphylococcus aureus type I signal peptidases.

Biophysical journal·2025

相关实验视频

Updated: Jan 12, 2026

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches
07:31

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches

Published on: September 1, 2023

3.1K

为大规模的膜蛋白仿真优化粗粒度模型.

Chen Yun Wen1, Yun Lyna Luo1, Jesper J Madsen2

  • 1Department of Biotechnology and Pharmaceutical Sciences, College of Pharmacy, Western University of Health Sciences, Pomona, California.

Biophysical reports
|November 8, 2025
PubMed
概括
此摘要是机器生成的。

我们优化了粗粒度 (CG) 脂质模型,用于大规模的膜模拟. 改进的模型增强了稳定性,使得在生理上相关的尺度上研究膜蛋白诱导的双层变形.

更多相关视频

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes
09:42

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes

Published on: January 16, 2016

9.4K
Incorporating Target Protein Structure Flexibility and Dynamics in Computational Drug Discovery Using Ensemble-Based Docking Analysis
08:49

Incorporating Target Protein Structure Flexibility and Dynamics in Computational Drug Discovery Using Ensemble-Based Docking Analysis

Published on: June 20, 2025

1.1K

相关实验视频

Last Updated: Jan 12, 2026

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches
07:31

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches

Published on: September 1, 2023

3.1K
Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes
09:42

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes

Published on: January 16, 2016

9.4K
Incorporating Target Protein Structure Flexibility and Dynamics in Computational Drug Discovery Using Ensemble-Based Docking Analysis
08:49

Incorporating Target Protein Structure Flexibility and Dynamics in Computational Drug Discovery Using Ensemble-Based Docking Analysis

Published on: June 20, 2025

1.1K

科学领域:

  • 生物物理学的生物物理.
  • 计算生物学 计算生物学
  • 材料科学 材料科学 材料科学

背景情况:

  • 粗粒度 (CG) 模型对于大规模模拟膜蛋白至关重要.
  • 在亚微米尺度上模拟由膜蛋白诱导的长距离双层变形在计算上具有挑战性.
  • 现有的通用无溶剂CG脂质模型在大型膜系统的稳定性方面存在局限性.

研究的目的:

  • 评估和优化一种通用的无溶剂CG脂质模型,用于膜蛋白的大规模分子动力学模拟.
  • 为了克服在以前的CG脂质模型中观察到的不稳定性问题 (膜孔,非物理波纹),超出了关键膜大小.
  • 为了能够研究由像PIEZO这样的膜蛋白诱导的双层变形,在具有不同机械性质的系统中.

主要方法:

  • 一种通用的无溶剂粗粒度 (CG) 脂质模型的系统优化.
  • 膜系统的大规模分子动力学模拟.
  • 评估模型稳定性随着膜尺寸的增加.
  • 模拟由机械敏感离子通道PIEZO诱导的膜变形.

主要成果:

  • 通用CG脂质模型表现出不稳定性 (孔隙,波纹) 超出了关键膜大小.
  • 系统的优化显著提高了模型的稳定性,用于更大的膜系统.
  • 优化的CG模型成功模拟了由PIEZO通道诱导的双层膜变形,具有可调节的机械特性.

结论:

  • 优化的CG脂质模型为大规模的膜模拟提供了增强的稳定性.
  • 这种改进的模型有助于研究双层介导的膜蛋白相互作用.
  • 该模型弥合了连续弹性理论和用于膜生物物理学研究的原子模拟之间的差距.