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Updated: Jan 12, 2026

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Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry
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高温驱动了Rhodosporidiobolus odoratus XQR中通过协调的转录基因-代谢重编程的托鲁拉罗丁主导型胡卜素过度积累
Die Zhao1, Chunji Li2, Nan Zeng1
1College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, People's Republic of China.
Journal of biotechnology
|November 8, 2025
概括
高温显著提高了Rhodosporidiobolus odoratus中的胡卜素产量,特别是torularhodin,通过调节关键基因. 这项研究揭示了潜在的代谢工程的温度驱动的胡卜素生成机制.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 代谢工程是代谢工程.
背景情况:
- 胡卜素是有价值的颜料,具有多种工业应用.
- Rhodosporidiobolus odoratus产生商业上重要的胡卜素,如β-胡卜素,烯和烯.
- 了解胡卜素生物合成调节对于优化产量至关重要.
研究的目的:
- 调查低 (10°C) 和高 (30°C) 温度对R. odoratus XQR.中胡卜素生物合成的影响.
- 阐明了依赖温度的胡卜素生产背后的分子机制.
- 确定代谢工程的潜在目标,以提高胡卜素产量.
主要方法:
- 在最佳 (20°C),低 (10°C) 和高 (30°C) 温度下培养R. odoratus XQR.
- 量化总类胡卜素和特定类胡卜素 (β-胡卜素,托鲁,托鲁拉霍丁).
- 测量反应性氧物种 (ROS),超氧化物脱酶 (SOD),催化酶 (CAT) 活性和总蛋白质.
- 分析涉及类和胡卜素生物合成的关键基因的表达.
主要成果:
- 高温 (30°C) 与对照组 (20°C) 相比,增加了约3倍的总胡卜素和约9倍的torularhodin.
- 低温 (10°C) 降低了总胡卜素,体积标位略有增加.
- 高温提高了ROS和SOD活性,减少了总蛋白质,并提高了关键生物合成基因 (HMGCS,hmgA,mvaD,crtYB,crtI,crtZ,crtA) 的调节.
结论:
- 温度显著影响R. odoratus XQR中的胡卜素生物合成,高温会促进过度积累,尤其是torularhodin.
- 类和胡卜素生物合成基因的升级,以及代谢变化,驱动了这种反应.
- 这些发现为温度驱动的胡卜素生成提供了机制性的见解,并突出了通过代谢工程优化胡卜素生产的目标.
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