通过使用定性和定量检测方法,研究商业猪肉收获期间沙门氏菌污染的动态
1Meat Science and Animal Biologicals Discovery (MSABD) Program, Department of Animal and Dairy Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA.
Journal of food protection
|November 8, 2025
概括
沙门氏菌污染在猪肉加工中很常见,尽管度很低. 这项研究开发了一种生物地图,以跟踪整个收获期间的存在,揭示流行程度因设施和地点而异.
科学领域:
- 食品安全 食品安全
- 微生物学 微生物学
- 兽医公共卫生 兽医公共卫生
背景情况:
- 动物是沙门氏菌等病原体的存储库,在肉类收获期间构成交叉污染风险.
- 在商业环境中验证抗微生物干预措施是具有挑战性的,因为复杂的环境.
研究的目的:
- 开发沙门氏菌生物图,用于评估商业猪肉采集期间病原体的存在和度.
- 确定关键控制点并评估干预措施在减少沙门氏菌污染方面的有效性.
主要方法:
- 在收获期间,从三个猪设施的9个地点收集了尸体抽样 (N=675).
- 使用Gene-Up Quant和Gene-Up SLM2试验,对量化和定性沙门氏菌的样本进行分析.
- 使用线性混合效应模型和ANOVA进行流行数据分析.
主要成果:
- 沙门氏菌患病率受到设施,位置及其相互作用的显著影响.
- 患病率在入口猪中最高,并且在外部清除后降低.
- 低量的定量结果限制了对度的统计分析,但定性数据显示存在广泛.
结论:
- 在整个猪肉收获过程中,即使度低,沙门氏菌污染也很普遍.
- 有效的检测系统对于开发和评估过程控制策略至关重要.
- 生物地图方法为收获期间的沙门氏菌分布提供了有价值的见解.
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